Wu Jing, Pan Lin, Jin Xueqin, Li Weihua, Li Hongbing, Chen Jianmao, Yang Wen
Laboratory Animal Center, Ningxia Medical University, Yinchuan, China.
The Affiliated Hospital, Ningxia Medical University, Yinchuan, China.
Acta Cir Bras. 2018 Mar;33(3):207-215. doi: 10.1590/s0102-865020180030000002.
To investigate whether oxymatrine (OMT) prevents hepatic fibrosis in rats by regulating liver transforming growth factor β1 (TGF-β1) level.
Hepatic fibrosis was induced in rats by thioacetamide (TAA). Blood was collected at the end of week 12 to determine the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glutathione (GSH). Changes in liver tissue were observed after hematoxylin-eosin (HE) staining.
Fibrosis was confirmed by Masson's collagen staining. Liver TGF-β1 level was determined by ELISA. OMT significantly reduced serum ALT and AST but increased GSH levels in rats with hepatic fibrosis. Moreover, it significantly improved liver histology in rats with TAA-induced hepatic fibrosis. It significantly decreased liver TGF-β1 level compared to that in the untreated group. It also significantly reduced collagen deposition in rats.
Oxymatrine is effective in protecting rats from thioacetamide-induced hepatic fibrosis by regulating TGF-β1 expression.
研究氧化苦参碱（OMT）是否通过调节肝脏转化生长因子β1（TGF-β1）水平来预防大鼠肝纤维化。
用硫代乙酰胺（TAA）诱导大鼠肝纤维化。在第12周结束时采集血液，测定丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和谷胱甘肽（GSH）水平。苏木精-伊红（HE）染色后观察肝组织变化。
通过Masson胶原染色确认纤维化。用酶联免疫吸附测定法（ELISA）测定肝脏TGF-β1水平。氧化苦参碱显著降低肝纤维化大鼠的血清ALT和AST水平，但提高GSH水平。此外，它显著改善了TAA诱导的肝纤维化大鼠的肝脏组织学。与未治疗组相比，它显著降低了肝脏TGF-β1水平。它还显著减少了大鼠的胶原沉积。
氧化苦参碱通过调节TGF-β1表达，有效保护大鼠免受硫代乙酰胺诱导的肝纤维化。
