Department of Animal Science, Texas A&M University, College Station, Texas, USA.
Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, Texas, USA.
Biol Reprod. 2018 Sep 1;99(3):611-628. doi: 10.1093/biolre/ioy085.
Ovine trophectoderm (oTr1) cells were used to investigate effects of epinephrine (EP), norepinephrine (NE), and dopamine (DA) on their proliferation, migration and adhesion, secretion of interferon tau (IFNT), and expression of genes for synthesis of polyamines and apoptosis. Expression of mRNAs for agmatinase (AGMAT), arginine decarboxylase (ADC), ornithine decarboxylase (ODC1), and solute carrier family 7 (SLC7A1) (cationic amino acid transporter, Y + system), member 1 increased (P < 0.05) in oTr1 cells in response to EP and DA. However, expression of SLC7A1 decreased at high doses of EP and expression of ADC mRNA by oTr1 cells decreased in response to 20 and 40 ng/ml NE, and 40 ng/ml DA. Migration of oTr1 cells increased in response to EP, DA, and NE after 48 h of treatment. However, proliferation of oTr1 cells was inhibited by 300 pg/ml EP after 96 h and DA at 20 and 100 ng/ml. EP increased adhesion of oTr1 cells. The secretion of IFNT increased in response to 300 pg/ml EP, 100 ng/ml NE and DA after 48 h and at 96 h, and both DA (40 ng/ml) and NE (100 ng/ml). Expression of mRNAs for apoptotic genes (caspase 3, cathpsin B, BCL2 associated X protein "bax," B-cell lymphoma 2 "bcl2," and proto-oncogene "cmyc") decreased (P < 0.05) in response to catecholamines, but DA did not affect (P < 0.05) expression of cMYC mRNA. These results indicate that catecholamines play important roles in conceptus development during the peri-implantation period of pregnancy through effects on synthesis of polyamines, secretion of IFNT, and expression of apoptotic genes by oTr1 cells.
绵羊滋养层细胞（oTr1）用于研究肾上腺素（EP）、去甲肾上腺素（NE）和多巴胺（DA）对其增殖、迁移和黏附、干扰素 tau（IFNT）分泌以及多胺合成和凋亡相关基因表达的影响。EP 和 DA 可引起 oTr1 细胞中 agmatinase（AGMAT）、精氨酸脱羧酶（ADC）、鸟氨酸脱羧酶 1（ODC1）和溶质载体家族 7（SLC7A1）（阳离子氨基酸转运体，Y+系统）成员 1 的 mRNA 表达增加（P<0.05）。然而，oTr1 细胞在高剂量 EP 下 SLC7A1 的表达降低，在 20 和 40ng/ml NE 和 40ng/ml DA 下 ADC mRNA 的表达降低。oTr1 细胞在 EP、DA 和 NE 处理 48 小时后迁移增加。然而，在 96 小时时，300pg/ml EP 抑制 oTr1 细胞的增殖，而 100ng/ml EP 和 DA 则抑制 oTr1 细胞的增殖。EP 增加 oTr1 细胞的黏附。IFNT 的分泌在 300pg/ml EP、100ng/ml NE 和 DA 处理 48 小时后增加，在 96 小时时也增加，而 DA（40ng/ml）和 NE（100ng/ml）也是如此。凋亡基因（caspase 3、cathpsin B、BCL2 相关 X 蛋白“bax”、B 细胞淋巴瘤 2“bcl2”和原癌基因“cmyc”）的 mRNA 表达在儿茶酚胺的作用下降低（P<0.05），但 DA 不影响（P<0.05）cMYC mRNA 的表达。这些结果表明，儿茶酚胺通过对 oTr1 细胞中多胺合成、IFNT 分泌和凋亡基因表达的影响，在妊娠着床期对胚胎发育起重要作用。
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