Gharibzadeh Farshad, Pourjam Ebrahim, Pedram Majid
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
J Nematol. 2018 Sep 3;50(2):207-218. doi: 10.21307/jofnem-2018-009.
Longidorus azarbaijanensis n. sp. is described and illustrated using morphological and molecular data. It was recovered in West Azarbaijan province, northwestern Iran, from the rhizospheric soil of foxtail weed. The new species is characterized by having 5.4 to 6.8 mm long females, offset, anteriorly flat lip region and separated from the rest of the body by a shallow constriction, funnel-shaped amphidial pouches, the guiding ring at 23 to 27 µm from the anterior end, 73 to 81 and 44 to 50 µm long odontostyle and odontophore, respectively, 95.0 to 113.5 µm long pharyngeal bulb, didelphic-amphidelphic reproductive system with long tubular uteri lacking sperm cells, vulva located at 52.0% to 56.4%, conical tail dorsally convex, ventrally flat, with bluntly rounded wide tip, for juvenile developmental stages and absence of males. The general body shape of the new species is similar to that of five known species of the genus namely L. euonymus, L. perangustus, L. persicus, L. protae and L. sturhani . The morphological differences of the new species with the aforementioned species are discussed. For all the aforementioned species (except L. protae, currently lacking molecular data) the differences of the new species was also confirmed with differences in molecular sequences of D2-D3 expansion domains of 28S rDNA and the corresponding phylogenetic analyses. The partial sequence of the internal transcribed spacer 1 (ITS1) of the new species was also used in phylogenetic analyses. In partial 28S tree, the clade including the new species and six other species ( L. attenuatus, Longidorus sp. and four above-mentioned species having molecular data for this fragment) was well supported in Bayesian inference. In the ITS1 tree, the new species formed a clade with L. euonymus , L. perangustus and L. persicus , as in 28S tree. This is one of the cases from which the morphologically similar species are separated using molecular sequences.
描述并阐释了新物种阿塞拜疆长针线虫（Longidorus azarbaijanensis n. sp.），使用了形态学和分子数据。它是在伊朗西北部的西阿塞拜疆省，从狐尾草的根际土壤中发现的。该新物种的特征为：雌虫体长5.4至6.8毫米，唇区偏移、前部扁平，与虫体其余部分由一浅缢缩分开，具漏斗形的侧器囊，引导环距前端23至27微米，口针和口针基部长度分别为73至81微米和44至50微米，咽球长95.0至113.5微米，双雌虫型生殖系统，子宫长管状且无精子，阴门位于虫体的52.0%至56.4%处，尾部呈圆锥形，背面凸起，腹面扁平，顶端宽且钝圆，有幼虫发育阶段且无雄虫。新物种的总体体型与该属五个已知物种即卫矛长针线虫（L. euonymus）、极细长针线虫（L. perangustus）、波斯长针线虫（L. persicus）、原长针线虫（L. protae）和施氏长针线虫（L. sturhani）相似。讨论了新物种与上述物种的形态差异。对于所有上述物种（除了原长针线虫，目前缺乏分子数据），新物种与它们在28S rDNA的D2-D3扩展域分子序列上的差异以及相应的系统发育分析也证实了这种差异。新物种的内部转录间隔区1（ITS1）部分序列也用于系统发育分析。在部分28S树中，包含新物种和其他六个物种（细弱长针线虫（L. attenuatus）、长针线虫属物种（Longidorus sp.）以及上述四个有该片段分子数据的物种）的分支在贝叶斯推断中得到了有力支持。在ITS1树中，新物种与卫矛长针线虫、极细长针线虫和波斯长针线虫形成一个分支，如同在28S树中一样。这是利用分子序列区分形态相似物种的案例之一。
