Department of Microbiology and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, United States.
Front Immunol. 2018 Apr 4;9:660. doi: 10.3389/fimmu.2018.00660. eCollection 2018.
The requirements for effector and memory CD8 T cell development are incompletely understood. Recent work has revealed a role for G-protein coupled receptor 18 (GPR18) in establishment of the intestinal CD8αα intraepithelial lymphocyte compartment. Here, we report that GPR18 is also functionally expressed in conventional CD8αβ T cells. When the receptor is lacking, mice develop fewer CD8 KLRG1 Granzyme B effector-memory cells. Bone marrow chimera studies show that the GPR18 requirement is CD8 T cell intrinsic. GPR18 is not required for T-bet expression in KLRG1 CD8 T cells. Gene transduction experiments confirm the functional activity of GPR18 in CD8 T cells. In summary, we describe a novel GPCR requirement for establishment or maintenance of the CD8 KLRG1 effector-memory T cell compartment. These findings have implications for methods to augment CD8 effector cell numbers.
效应器和记忆 CD8 T 细胞发育的要求尚不完全清楚。最近的工作揭示了 G 蛋白偶联受体 18（GPR18）在建立肠道 CD8αα 上皮内淋巴细胞区室中的作用。在这里，我们报告 GPR18 也在常规 CD8αβ T 细胞中具有功能表达。当受体缺失时，小鼠中 CD8 KLRG1 颗粒酶 B 效应记忆细胞的数量减少。骨髓嵌合体研究表明，GPR18 是 CD8 T 细胞内在的需求。GPR18 不要求 KLRG1 CD8 T 细胞中 T 细胞生成素的表达。基因转导实验证实了 GPR18 在 CD8 T 细胞中的功能活性。总之，我们描述了一种新型 GPCR 对建立或维持 CD8 KLRG1 效应记忆 T 细胞区室的要求。这些发现对增强 CD8 效应细胞数量的方法具有重要意义。
