Department of Health Technology and Informatics, Hong Kong Polytechnic University, Kowloon, Hong Kong.
School of Biomedical Sciences, Chinese University of Hong Kong, New Territories, Hong Kong.
Oxid Med Cell Longev. 2018 Feb 12;2018:8267560. doi: 10.1155/2018/8267560. eCollection 2018.
Danshensu (DSS) is an active ingredient extracted from the root of the Danshen that could ameliorate oxidative stress via upregulation of heme oxygenase- (HO-) 1. Little is known about the treatment effects of DSS on kidney function in diabetic mice. Therefore, the primary aim of the present study was to characterize the renal clearance kinetics of IRdye800CW in mice after DSS treatment. The secondary aim was to measure several biomarkers of renal function and oxidative stress (urinary F2-isoprostane, HO-1 in kidney and serum bilirubin). Fourteen diabetic mice were randomly assigned into two groups and received either DSS treatment (DM + DSS) or vehicle treatment (DM). A third group that comprised of nondiabetic mice (non-DM control) received no DSS treatment and served as the nondiabetic control. At the end of a 3-week intervention period, serum and urinary biomarkers of renal function and oxidative stress were assessed and the renal clearance of IRdye800CW dye in all mice was determined noninvasively using Multispectral Optoacoustic Tomography. The major finding from this study suggested that DSS treatment in mice improved renal clearance. Increased expression of HO-1 after DSS treatment also suggested that DSS might represent a potential therapeutic avenue for clinical intervention in diabetic nephropathy.
丹参素（DSS）是从丹参根中提取的一种活性成分，可通过上调血红素加氧酶-1（HO-1）来改善氧化应激。关于 DSS 对糖尿病小鼠肾功能的治疗作用知之甚少。因此，本研究的主要目的是描述 DSS 处理后 小鼠中 IRdye800CW 的肾脏清除动力学。次要目的是测量肾功能和氧化应激的几个生物标志物（尿 F2-异前列烷、肾脏和血清胆红素中的 HO-1）。14 只糖尿病小鼠被随机分为两组，分别接受 DSS 治疗（DM+DSS）或载体治疗（DM）。第三组由非糖尿病小鼠（非 DM 对照组）组成，未接受 DSS 处理，作为非糖尿病对照组。在 3 周干预期结束时，评估血清和尿液肾功能和氧化应激生物标志物，并使用多光谱光声断层扫描术非侵入性地确定所有小鼠的 IRdye800CW 染料的肾脏清除率。这项研究的主要发现表明，DSS 处理改善了 小鼠的肾脏清除率。DSS 处理后 HO-1 的表达增加表明，DSS 可能代表了糖尿病肾病临床干预的一种潜在治疗途径。
