袖状胃切除术对 GK 大鼠糖代谢及胰高血糖素样肽 1 的影响

The Effects of Sleeve Gastrectomy on Glucose Metabolism and Glucagon-Like Peptide 1 in Goto-Kakizaki Rats.

机构信息

Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Guangdong Institute of Gastroenterology, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, Guangzhou, China.

出版信息

J Diabetes Res. 2018 Feb 18;2018:1082561. doi: 10.1155/2018/1082561. eCollection 2018.

PURPOSE

To investigate the effects of sleeve gastrectomy (SG) on glucose metabolism and changes in glucagon-like peptide 1 (GLP-1) in Goto-Kakizaki (GK) rats.

METHODS

GK rats were randomly assigned to one of three groups: SG, SG pair-fed plus sham surgery (PF-sham), and ad libitum-fed no surgery (control). Food intake, body weight, blood glucose, GLP-1 and insulin levels, and GLP-1 expression in the jejunum and ileum were compared.

RESULTS

The SG rats exhibited lower postoperative food intake, body weight, and fasting glucose than did the control rats ( < 0.05). SG significantly improved glucose and insulin tolerance ( < 0.05). Plasma GLP-1 levels were higher in SG rats than in control or PF-sham rats in the oral glucose tolerance test (OGTT) ( < 0.05). Blood glucose levels expressed as a percentage of baseline were higher in SG rats than in control rats after exendin (9-39) administration ( < 0.05). The levels of GLP-1 expression in the jejunum and ileum were higher in SG rats than in PF-sham and control rats ( < 0.05).

CONCLUSIONS

Improvement of glucose metabolism by SG was associated with increased GLP-1 secretion. SG contributes to an increase in plasma GLP-1 levels via increased GLP-1 expression in the mucosa of the jejunum and/or ileum.

目的

研究袖状胃切除术（SG）对葡萄糖代谢和胰高血糖素样肽 1（GLP-1）变化的影响。

方法

GK 大鼠随机分为 3 组：SG 组、SG 配对喂养加假手术（PF-sham）组和自由喂养无手术（对照）组。比较食物摄入量、体重、血糖、GLP-1 和胰岛素水平以及空肠和回肠的 GLP-1 表达。

结果

SG 大鼠术后食物摄入量、体重和空腹血糖均低于对照组（<0.05）。SG 显著改善了葡萄糖和胰岛素耐量（<0.05）。在口服葡萄糖耐量试验（OGTT）中，SG 大鼠的血浆 GLP-1 水平高于对照组或 PF-sham 大鼠（<0.05）。给予外源性 GLP-1 类似物 exendin（9-39）后，SG 大鼠的血糖水平较对照组大鼠的血糖水平更高（<0.05）。SG 大鼠空肠和回肠的 GLP-1 表达水平高于 PF-sham 组和对照组大鼠（<0.05）。