Akabane S, Kojima S, Kawamura M, Iida T, Matsushima Y, Ito K
Department of Cardiovascular Dynamics, National Cardiovascular Center, Osaka, Japan.
Clin Exp Hypertens A. 1988;10(2):311-22. doi: 10.3109/10641968809103529.
To assess the released form of atrial natriuretic peptide (ANP) during atrial distension, we compared the form of ANP before and during atrial distension in anesthetized dogs, using radioimmunoassay combined with high performance liquid chromatography (HPLC). When increasing the left atrial pressure (LAP) from 2.2 +/- 0.9 mmHg to 9.4 +/- 1.3 mmHg, the plasma ANP concentration in the coronary sinus was increased by 190% over the control, during left atrial distension. The major form of the released ANP extracted in the blood sample obtained from the coronary sinus was alpha-form. However other unidentified immunoreactive peaks, preceding and following the main peak were also discernible. These results suggest that the major form of ANP released during left atrial distension was the alpha-form.
为了评估心房扩张过程中心房利钠肽（ANP）的释放形式，我们使用放射免疫分析法结合高效液相色谱法（HPLC），比较了麻醉犬心房扩张前后ANP的形式。当左心房压力（LAP）从2.2±0.9 mmHg增加到9.4±1.3 mmHg时，在左心房扩张期间，冠状窦血浆ANP浓度比对照增加了190%。从冠状窦采集的血样中提取的释放ANP的主要形式是α形式。然而，在主峰之前和之后的其他未鉴定的免疫反应峰也清晰可见。这些结果表明，左心房扩张期间释放的ANP的主要形式是α形式。
