Gnaiger E
Experientia Suppl. 1979;37:155-65. doi: 10.1007/978-3-0348-5545-7_23.
The measurement of heat production represents the most general approach to the estimation of energy flow through biological systems. While aerobic energy metabolism is most conveniently studied by polarographic oxygen determination, direct calorimetry presents the only unspecific method for quantitative comparison of aerobic and anoxic metabolism in animals. In flow calorimeters constant experimental conditions may be controlled for practically unlimited periods of time, and transitions of the environmental regime may be repeatedly performed during one experiment. The merits of a direct calorimetric flow system are demonstrated in case studies of anoxic and aerobic animal metabolism and discussed in the context of current biochemical and ecophysiological concepts.
热量产生的测量是估算生物系统能量流动的最常用方法。虽然通过极谱法测定氧气来研究有氧能量代谢最为便捷，但直接量热法是对动物有氧和无氧代谢进行定量比较的唯一非特异性方法。在流动量热计中，可以在几乎无限长的时间内控制恒定的实验条件，并且在一个实验过程中可以反复进行环境状态的转变。直接量热流动系统的优点在动物无氧和有氧代谢的案例研究中得到了证明，并在当前生化和生态生理学概念的背景下进行了讨论。
