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利用酸性条件下剩余的大豆油对酵母生物表面活性剂进行表征及其在金属去除过程中的应用。

Characterization of biosurfactant from yeast using residual soybean oil under acidic conditions and their use in metal removal processes.

机构信息

Graduate Program in Biotechnology and Environmental Monitoring, Federal University of São Carlos- UFSCar, João Leme dos Santos Highway, Km 101, 18052-780, Sorocaba, São Paulo, Brazil.

Graduate Program in Material Sciences, Universidade Federal de São Carlos UFSCar, Rod. João Leme dos Santos, Km 101, 18052-780, Sorocaba, São Paulo, Brazil.

出版信息

FEMS Microbiol Lett. 2018 May 1;365(10). doi: 10.1093/femsle/fny098.

Abstract

This study aimed at the production of biosurfactants from yeasts under acidic conditions using residual soybean oil as a carbon source, as well as the biosurfactant produced in the solubilization of metals in sewage sludge. The yeast Meyerozyma guilliermondii was considered the best producer in both pH 4.0 and 2.0; therefore, the product obtained by this yeast was characterized by Fourier transform infrared (FT-IR) and 1H nuclear magnetic resonance (NMR) spectroscopy. Moreover, it was applied in metal removal assays in anaerobic sewage sludge. The spectra obtained in FT-IR suggested that M. guilliermondii's biosurfactant had a similar structure to glycolipids from the sophorolipid class, and it was confirmed by 1H NMR spectroscopy. In the bioleaching assays, the application of biosurfactant (2%) produced by M. guilliermondii with pH adjusted to 2.0 was able to solubilize 15.9% of cadmium from the sewage sludge.

摘要

本研究旨在利用剩余的大豆油作为碳源,在酸性条件下从酵母中生产生物表面活性剂,以及利用生物表面活性剂从污水污泥中溶解金属。在 pH 值为 4.0 和 2.0 时,酵母 Meyerozyma guilliermondii 被认为是最佳的生产者;因此,通过该酵母获得的产物通过傅里叶变换红外(FT-IR)和 1H 核磁共振(NMR)光谱进行了表征。此外,它还应用于厌氧污水污泥中的金属去除试验中。FT-IR 获得的光谱表明,M. guilliermondii 的生物表面活性剂具有类似于糖脂类鞘脂的结构,这通过 1H NMR 光谱得到了证实。在生物沥滤试验中,将 pH 值调节至 2.0 的 M. guilliermondii 产生的生物表面活性剂(2%)能够从污水污泥中溶解 15.9%的镉。

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