Department of Orthodontics, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Biomaterials, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
J Biomed Mater Res A. 2018 Sep;106(9):2472-2480. doi: 10.1002/jbm.a.36442. Epub 2018 May 14.
Osteoporosis is a major cause of age-related fractures. Healing complications in osteoporotic patients are often associated with increased mortality and morbidity. Stimulation of the implant-adjacent bone could be beneficial in terms of the surgical outcome. Over the past decade, numerous investigations have implicated insulin in normal bone growth, and recent studies have described the advantages of administering insulin locally to increase bone formation. Therefore, we hypothesized that insulin-coated titanium implants would increase bone formation in osteoporotic animals. The aim of this study was to evaluate the effects of insulin delivered from an implant surface on bone-related gene expression and bone formation in osteoporotic rats. Characterizations of the surfaces of insulin-coated and control implants were performed using ellipsometry and interferometry. Forty ovariectomized and four healthy Sprague Dawley rats were used and implants were inserted in the tibias. The systemic effect of insulin was assessed by measuring the blood glucose levels and total body weight. The animals were sacrificed either 1 day or 3 weeks postimplantation. Implant-adherent cells were analyzed by quantitative real-time PCR, and the bone adjacent to the implants was examined by microcomputed tomography and histomorphometry. The insulin-coated implants had no systemic effects. The insulin-coated samples demonstrated significantly lower expression of the gene for interleukin 1β (p = 0.019) at 1 day, and significantly exhibited more periosteal callus (p = 0.029) at 3 weeks. Locally delivered insulin has potential for promoting bone formation and it exerts potentially anti-inflammatory effects in osteoporotic rats. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2472-2480, 2018.
骨质疏松症是与年龄相关的骨折的主要原因。骨质疏松症患者的愈合并发症通常与死亡率和发病率增加有关。刺激植入物相邻的骨骼在手术结果方面可能是有益的。在过去的十年中，许多研究表明胰岛素在正常骨骼生长中起作用，最近的研究描述了局部给予胰岛素以增加骨形成的优势。因此，我们假设胰岛素涂层钛植入物会增加骨质疏松动物的骨形成。本研究旨在评估从植入物表面释放的胰岛素对骨质疏松症大鼠的骨相关基因表达和骨形成的影响。使用椭圆偏振术和干涉测量法对胰岛素涂层和对照植入物的表面特性进行了表征。使用 40 只卵巢切除术和 4 只健康的 Sprague Dawley 大鼠，并将植入物插入胫骨中。通过测量血糖水平和总体重来评估胰岛素的全身作用。植入后 1 天或 3 周处死动物。通过定量实时 PCR 分析附着在植入物上的细胞，通过微计算机断层扫描和组织形态计量学检查植入物相邻的骨骼。胰岛素涂层植入物没有全身作用。在第 1 天，胰岛素涂层样品的白细胞介素 1β基因表达明显降低（p = 0.019），在第 3 周，骨膜骨痂明显增加（p = 0.029）。局部递送的胰岛素具有促进骨形成的潜力，并在骨质疏松症大鼠中发挥潜在的抗炎作用。©2018 Wiley Periodicals，Inc. J Biomed Mater Res Part A：106A：2472-2480，2018。
