Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
J Biomed Mater Res A. 2013 Jan;101(1):132-7. doi: 10.1002/jbm.a.34313. Epub 2012 Jul 24.
The possibility to control bone formation would be favorable in many areas of medicine, where bone defects is still a major challenge. Insulin has been suggested to exert both systemic and local anabolic effects in bone tissues. This raised the question whether locally administrated insulin could provide new therapeutic strategies for patients with local bone defects and impaired bone healing. The aim of this study was to evaluate bone formation in non-diabetic rats when local insulin is administered. This study differs from previous reports in two aspects: the use of non-diabetic animals and locally administered insulin. Twenty-four implants were inserted into 12 rats-one insulin-coated and one control-in each tibia for four weeks. Interferometry and histomorphometry were used to evaluate the surface topography and bone formation, respectively. Results demonstrated no significant changes in surface topography after insulin immobilization. Histomorphometry revealed significantly more bone around the insulin-coated implants (BA) (p = 0.005) and a similar amount of bone at the implant surface (BIC) (p = 0.117) compared with the controls. It was concluded that locally administered insulin from a titanium implant surface has the potential to increase bone formation not only in diabetic subjects but also in non-diabetic subjects.
在许多医学领域，控制骨形成的可能性是有利的，因为骨缺损仍然是一个主要挑战。胰岛素被认为对骨组织具有全身和局部合成代谢作用。这就提出了一个问题，即局部给予胰岛素是否可以为局部骨缺损和骨愈合受损的患者提供新的治疗策略。本研究旨在评估局部给予胰岛素时非糖尿病大鼠的骨形成情况。这项研究与以前的报告在两个方面有所不同：使用非糖尿病动物和局部给予胰岛素。在每只胫骨中，将 24 个植入物分别植入 12 只大鼠中的一只（胰岛素涂层和一只对照），持续四周。干涉测量法和组织形态计量学分别用于评估表面形貌和骨形成。结果表明，胰岛素固定后表面形貌没有明显变化。组织形态计量学显示，与对照组相比，胰岛素涂层植入物周围的骨量明显增加（BA）（p=0.005），而植入物表面的骨量相似（BIC）（p=0.117）。结论是，从钛植入物表面局部给予胰岛素具有增加骨形成的潜力，不仅在糖尿病患者中，而且在非糖尿病患者中也是如此。
