Allelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), Campus de Excelencia Internacional (ceiA3), School of Science , University of Cadiz , Calle República Saharaui no. 7 , 11510 Puerto Real , Cadiz , Spain.
J Agric Food Chem. 2018 May 9;66(18):4638-4644. doi: 10.1021/acs.jafc.8b01501. Epub 2018 Apr 25.
Stigmasterol, bergapten, and α-tomatine were isolated from tomato roots. The preliminary phytotoxic activities of stigmasterol and α-tomatine were evaluated in a wheat-coleoptile bioassay, and α-tomatine was more active than stigmasterol. To confirm its phytotoxic activity, α-tomatine was tested on Lactuca sativa and two weeds ( Lolium perenne and Echinochloa crus-galli), and it was active in all cases. The stimulatory activities of α-tomatine and stigmasterol on parasitic-plant germination were also evaluated, and α-tomatine was found to be active on Phelipanche ramosa, a parasitic plant of tomato. α-Tomatine was identified in root exudates by LC-MS/MS. This confirms that α-tomatine is exuded by roots into the environment, where it could act as both an allelochemical and a stimulator of P. ramosa, a parasitic plant of tomato.
豆甾醇、佛手柑内酯和α-茄碱从番茄根部分离得到。在小麦胚芽生物测定中评价了豆甾醇和α-茄碱的初步植物毒性活性，结果表明α-茄碱比豆甾醇更具活性。为了确认其植物毒性活性，对α-茄碱进行了莴苣和两种杂草（黑麦草和稗草）的测试，在所有情况下均具有活性。还评估了α-茄碱和豆甾醇对寄生植物发芽的刺激活性，结果表明α-茄碱对番茄的寄生植物 Phelipanche ramosa 具有活性。通过 LC-MS/MS 在根分泌物中鉴定出α-茄碱。这证实了α-茄碱是从根部分泌到环境中的，在环境中它既可以作为化感物质，也可以作为番茄寄生植物 P. ramosa 的刺激物。
