Xu Xia, DU Huan, Wu Yalan, Cheng Hui, Cao Jian, Meng Qingwei, Guo Mengyuan
College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.
Key Laboratory of Xin'an Medicine of Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Feb;34(2):110-114.
Objective To investigate the effects of modified Cheng's Juanbi Decoction (CJBD) on the level of cAMP in T lymphocytes and CD4/CD8 T cell ratio among rats with adjuvant arthritis (AA). Methods Male adult SD rats were randomly divided into normal control group, AA group, CJBD group, and tripterygium glycosides tablet (TGT) group. Blowing/cold water and Freund's complete adjuvant were used to establish a rat model of AA with wind-cold-dampness arthralgia. The control group was given normal saline by gavage, and the CJBD group and TGT group were given respective therapies. The course of treatment was 7 days for all groups. Blood was collected from the orbit, and peripheral blood mononuclear cells (PBMCs) were isolated. Magnetic activated cell sorting was used to separate T lymphocytes. A cAMP detection kit was used to measure the cAMP levels in T lymphocytes. Flow cytometry was performed to determine the numbers of CD4 and CD8 T cells in peripheral T cells, and the CD4/CD8 T cell ratio was calculated. Results Compared with the normal control group, the CJBD group had significantly reduced expression of cAMP in T lymphocytes, but there was no significant difference in cAMP level between the TGT group and the normal control group. The CJBD group had a significantly lower CD4/CD8 T cell ratio than the normal control group. Conclusion CJBD can reduce the cAMP level in T lymphocytes and CD4/CD8 T cell ratio in rats with AA.
目的 探讨加味成氏蠲痹汤（CJBD）对佐剂性关节炎（AA）大鼠T淋巴细胞中环磷酸腺苷（cAMP）水平及CD4/CD8 T细胞比值的影响。方法 将成年雄性SD大鼠随机分为正常对照组、AA组、CJBD组和雷公藤多苷片（TGT）组。采用吹风/冷水刺激和弗氏完全佐剂建立风寒湿痹型AA大鼠模型。对照组给予生理盐水灌胃，CJBD组和TGT组给予相应治疗。所有组治疗疗程均为7天。通过眼眶取血，分离外周血单个核细胞（PBMCs）。采用磁珠分选法分离T淋巴细胞。使用cAMP检测试剂盒检测T淋巴细胞中的cAMP水平。采用流式细胞术检测外周T细胞中CD4和CD8 T细胞数量，并计算CD4/CD8 T细胞比值。结果 与正常对照组相比，CJBD组T淋巴细胞中cAMP表达显著降低，但TGT组与正常对照组之间cAMP水平无显著差异。CJBD组CD4/CD8 T细胞比值显著低于正常对照组。结论 CJBD可降低AA大鼠T淋巴细胞中的cAMP水平及CD4/CD8 T细胞比值。
