Franch A, Castellote C, Castell M
Unitat de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Ann Rheum Dis. 1994 Jul;53(7):461-6. doi: 10.1136/ard.53.7.461.
To determine the phenotype of peripheral blood lymphocytes during the time-course of adjuvant arthritis (AA) to detect alterations that could be involved in the pathogenesis of the arthritic process.
Phenotype analysis was performed on days 7, 14, 21, 28, 42, 56 and 70 after arthritis induction using monoclonal antibodies to CD5, CD4 and CD8 subsets, and flow cytometry. The proportion of activated lymphocytes and lymphocytes was also assessed with monoclonal antibodies to IL-2R (CD25), to Ia antigen and by polyclonal antibodies to rat Ig.
Adjuvant arthritis produced leukocytosis with neutrophilia. Rats with AA showed a marked increase in the number of both CD4+ and CD8+ cells. The ratio CD4/CD8 decreased because the rise in CD8+ cells was more pronounced than the increase in CD4+ cells. Changes in lymphocyte counts showed two well-defined periods: the first, from day 14 to day 28, during which the inflammation of the joints reached a maximum and changes in lymphocyte subsets were more pronounced, that is, there was a threefold increase in CD8+ lymphocytes over normal counts, and the second, from day 42 to day 70, in which modified parameters improved considerably but remained different from controls.
Alterations were detected in the phenotype of peripheral blood lymphocytes in AA, which provides an additional marker of disease activity.
确定佐剂性关节炎（AA）病程中外周血淋巴细胞的表型，以检测可能参与关节炎发病机制的改变。
在诱导关节炎后的第7、14、21、28、42、56和70天，使用针对CD5、CD4和CD8亚群的单克隆抗体及流式细胞术进行表型分析。还使用针对IL-2R（CD25）、Ia抗原的单克隆抗体以及针对大鼠Ig的多克隆抗体评估活化淋巴细胞和淋巴细胞的比例。
佐剂性关节炎导致白细胞增多伴中性粒细胞增多。患AA的大鼠CD4+和CD8+细胞数量均显著增加。CD4/CD8比值降低，因为CD8+细胞的增加比CD4+细胞的增加更为明显。淋巴细胞计数的变化显示出两个明确的时期：第一个时期是从第14天到第28天，在此期间关节炎症达到最大值，淋巴细胞亚群的变化更为明显，即CD8+淋巴细胞比正常计数增加了三倍；第二个时期是从第42天到第70天，在此期间各项参数虽有明显改善，但仍与对照组不同。
在AA中检测到外周血淋巴细胞表型的改变，这为疾病活动提供了一个额外的标志物。
