School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China.
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China.
Talanta. 2018 Jul 1;184:307-315. doi: 10.1016/j.talanta.2018.03.010. Epub 2018 Mar 9.
In present study, novel magnetic molecularly imprinted polymers for hesperetin were successfully prepared by surface molecular imprinting method using functionalized FeO particles as the magnetic cores. Hesperetin as the template, N-Isopropylacrylamide as the functional monomer, ethylene glycol dimethyl acrylate as the crosslinker, 2,2-azobisisobutyonnitrile as initiator and acetonitrile-methanol (3:1, v/v) as the porogen were applied in the preparation process. Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscope, x-ray diffraction and vibrating sample magnetometry were applied to characterize the magnetic molecularly imprinted polymers. The adsorption experiments indicated that the magnetic molecularly imprinted polymers performed high selective recognition property to hesperetin. The selectivity experiment indicated that the adsorption capacity and selectivity of polymers to hesperetin was higher than that of luteolin, baicalein and ombuin. Furthermore, the magnetic molecularly imprinted polymers were employed as adsorbents for extraction and enrichment of hesperetin from the dried pericarp of Citrus reticulata Blanco. The recoveries of hesperetin in the dried pericarp of Citrus reticulata Blanco ranged from 90.5% to 96.9%. The linear range of 0.15-110.72 µg/mL was obtained with correlation coefficient of greater than 0.9991. The limit of detection and quantification of the proposed method was 0.06 µg/mL and 0.15 µg/mL, respectively. Based on three replicate measurements, intra-day RSD was 0.71% and inter-day RSD was 2.31%. These results demonstrated that the prepared magnetic molecularly imprinted polymers were proven to be an effective material for the selective adsorption and enrichment of hesperetin from natural medicines, fruits and et al.
在本研究中，通过表面分子印迹法成功制备了用于橙皮素的新型磁性分子印迹聚合物，该方法使用功能化的 FeO 颗粒作为磁性核。橙皮素作为模板，N-异丙基丙烯酰胺作为功能单体，乙二醇二甲基丙烯酸酯作为交联剂，2,2-偶氮二异丁腈作为引发剂，乙腈-甲醇（3:1，v/v）作为致孔剂应用于制备过程中。傅里叶变换红外光谱、扫描电子显微镜、透射电子显微镜、X 射线衍射和振动样品磁强计用于表征磁性分子印迹聚合物。吸附实验表明，磁性分子印迹聚合物对橙皮素有高选择性识别性能。选择性实验表明，聚合物对橙皮素的吸附容量和选择性高于芦丁、黄芩素和橙皮素。此外，磁性分子印迹聚合物被用作从干燥的陈皮中提取和富集橙皮素的吸附剂。橙皮苷在干燥的陈皮中的回收率在 90.5%至 96.9%之间。在 0.15-110.72µg/mL 范围内获得了大于 0.9991 的相关系数。该方法的检测限和定量限分别为 0.06µg/mL 和 0.15µg/mL。基于三次重复测量，日内 RSD 为 0.71%，日间 RSD 为 2.31%。这些结果表明，所制备的磁性分子印迹聚合物被证明是一种从天然药物、水果等中选择性吸附和富集橙皮素的有效材料。
