Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
Talanta. 2018 Jul 1;184:491-498. doi: 10.1016/j.talanta.2018.03.046. Epub 2018 Mar 16.
The covalent modification of polymeric particles has been a challenge due to their chemical inertness. Herein we describe a facile and neat method via thiol-ene reaction to immobilize cysteamine/cysteine onto allylmethacrylate-divinylbenzene (AMA-DVB) and ethylvinylbenzene-divinylbenzene (EVB-DVB). The introduced functionalities were verified by elementary analysis and scanning electron microscope coupled with energy dispersive spectroscopy. This modification enabled the further grafting of hyperbranched condensation polymers (HBCPs), which converted the particles into anion exchangers. The anion exchangers demonstrated good separation with carbonate/bicarbonate eluents and hydroxide eluents for suppressed anion exchange chromatography. In the case of a 3-layered anion exchanger, simultaneous separation of nine model anions was achieved by isocratic elution in 17 min with efficiencies up to 16,100 plates m. High stability and dependable durability was exhibited in long-term tests.
由于聚合粒子的化学惰性，其共价修饰一直是一个挑战。在此，我们通过硫醇-烯反应描述了一种简便而整洁的方法，将半胱胺/半胱氨酸固定在丙烯酰甲基二乙烯基苯（AMA-DVB）和乙烯基苯二乙烯基苯（EVB-DVB）上。通过元素分析和扫描电子显微镜结合能谱对引入的官能团进行了验证。这种修饰使超支化缩聚物（HBCPs）的进一步接枝成为可能，从而将粒子转化为阴离子交换剂。阴离子交换剂在碳酸盐/碳酸氢盐洗脱液和氢氧化物洗脱液中表现出良好的分离性能，用于抑制型阴离子交换色谱。在 3 层阴离子交换剂的情况下，通过在 17 分钟内等度洗脱，可实现 9 种模型阴离子的同时分离，效率高达 16100 板/米。在长期测试中表现出高稳定性和可靠的耐用性。
