Pan Shaojie, Lou Chaoyan, Yu Xiaolin, Zhang Kaidi, Zhang Kai, Jiang Lei, Zhu Yan
College of Quality and Standardization, China Jiliang University, Hangzhou 310018, China.
Ningbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Ningbo 315300, China.
Foods. 2025 Jun 28;14(13):2301. doi: 10.3390/foods14132301.
The migration of hindered phenolic antioxidants from food contact materials (FCMs) into foodstuffs poses health risks due to endocrine disruption and organ toxicity. Hence, the development of a high-efficiency analytical method for hindered phenolic antioxidants is of great importance for food safety. This study developed a novel ultrasound-assisted dispersive solid-phase filter extraction (d-SPFE) coupled with green supercritical fluid chromatography (SFC) method for the simultaneous determination of six representative hindered phenolic antioxidants. Under optimized conditions, the method achieved high extraction efficiency, with the complete separation of all analytes within 10 min. A wide linearity range (0.02-2.0 μg/mL) was achieved, with coefficients of determination all greater than 0.9996. The limits of detection (LOD, S/N = 3) and limits of quantification (LOQ, S/N = 10) were 2.4-3.6 ng/mL and 8-12 ng/mL, respectively. Validation tests demonstrated precise spiked recoveries (89.4-101.6%), with intra-day and inter-day relative standard deviations (RSDs) all less than 10%. The d-SPFE-SFC synergy significantly outperforms conventional techniques in terms of analysis speed and eco-efficiency. Successful application to food simulants confirms its reliability in monitoring hindered phenolic antioxidant migration from FCMs. This green and rapid methodology will enable the direct assessment of migration risks.
受阻酚类抗氧化剂从食品接触材料（FCMs）迁移到食品中会因内分泌干扰和器官毒性而带来健康风险。因此，开发一种高效的受阻酚类抗氧化剂分析方法对于食品安全至关重要。本研究开发了一种新型的超声辅助分散固相过滤萃取（d-SPFE）结合绿色超临界流体色谱（SFC）方法，用于同时测定六种代表性受阻酚类抗氧化剂。在优化条件下，该方法具有较高的萃取效率，所有分析物在10分钟内实现完全分离。线性范围宽（0.02 - 2.0 μg/mL），测定系数均大于0.9996。检测限（LOD，S/N = 3）和定量限（LOQ，S/N = 10）分别为2.4 - 3.6 ng/mL和8 - 12 ng/mL。验证试验表明加标回收率精确（89.4 - 101.6%），日内和日间相对标准偏差（RSDs）均小于10%。d-SPFE-SFC协同作用在分析速度和生态效率方面显著优于传统技术。成功应用于食品模拟物证实了其在监测FCMs中受阻酚类抗氧化剂迁移方面的可靠性。这种绿色快速的方法将能够直接评估迁移风险。
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