Cernacek P, Maher E, Crawhall J C, Levy M
Department of Medicine, McGill University, Royal Victoria Hospital, Montreal, Canada.
Life Sci. 1988;42(24):2533-9. doi: 10.1016/0024-3205(88)90353-0.
The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP99-126, thus confirming the structural identity of dog and human ANP.
通过放射免疫测定法测定的免疫反应性心钠素（iANP）水平在犬心房中呈现出相当均匀的分布。然而，在左、右心房的心耳中发现了最高浓度，并且在所有分析的部位，左侧的水平显著更高。心房提取物的反相高效液相色谱结合放射免疫测定法显示存在三种iANP组分。主要组分（占iANP的65%）估计分子量为16,300，表明它对应于激素前体。相比之下，在血浆提取物中，92%的iANP与合成的人ANP99 - 126共洗脱，从而证实了犬和人ANP的结构同一性。
