Gryganskyi Andrii P, Golan Jacob, Dolatabadi Somayeh, Mondo Stephen, Robb Sofia, Idnurm Alexander, Muszewska Anna, Steczkiewicz Kamil, Masonjones Sawyer, Liao Hui-Ling, Gajdeczka Michael T, Anike Felicia, Vuek Antonina, Anishchenko Iryna M, Voigt Kerstin, de Hoog G Sybren, Smith Matthew E, Heitman Joseph, Vilgalys Rytas, Stajich Jason E
Department of Biology, Duke University, Durham, North Carolina, 27708
Department of Botany and Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, 53706.
G3 (Bethesda). 2018 May 31;8(6):2007-2018. doi: 10.1534/g3.118.200235.
Phylogenomic approaches have the potential to improve confidence about the inter-relationships of species in the order Mucorales within the fungal tree of life. species are especially important as plant and animal pathogens and bioindustrial fermenters for food and metabolite production. A dataset of 192 orthologous genes was used to construct a phylogenetic tree of 21 strains, classified into four species isolated from habitats of industrial, medical and environmental importance. The phylogeny indicates that the genus consists of three major clades, with as the basal species and the sister lineage to and two closely related species and A comparative analysis of the mating type locus across reveals that its structure is flexible even between different species in the same genus, but shows similarities between and other mucoralean fungi. The topology of single-gene phylogenies built for two genes involved in mating is similar to the phylogenomic tree. Comparison of the total length of the genome assemblies showed that genome size varies by as much as threefold within a species and is driven by changes in transposable element copy numbers and genome duplications.
系统发育基因组学方法有潜力提高对真菌生命之树中毛霉目物种间相互关系的确信度。毛霉目物种作为植物和动物病原体以及用于食品和代谢产物生产的生物工业发酵菌尤为重要。一个包含192个直系同源基因的数据集被用于构建21个菌株的系统发育树，这些菌株被分为从具有工业、医学和环境重要性的栖息地分离出的四个物种。系统发育分析表明，该属由三个主要分支组成，其中[物种名称1]作为基部物种，是[物种名称2]和两个密切相关物种[物种名称3]和[物种名称4]的姐妹谱系。对整个[属名]的交配型位点进行比较分析发现，即使在同一属的不同物种之间，其结构也是灵活的，但在[物种名称5]和其他毛霉目真菌之间表现出相似性。为参与交配的两个基因构建的单基因系统发育树的拓扑结构与系统发育基因组树相似。基因组组装总长度的比较表明，一个物种内的基因组大小变化可达三倍之多，这是由转座元件拷贝数的变化和基因组加倍驱动的。
