Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL, 60153, USA.
Host-Microbiota Interactions Laboratory, Wellcome Sanger Institute, Hinxton, CB10 1SA, UK.
Nat Commun. 2018 Apr 19;9(1):1557. doi: 10.1038/s41467-018-03968-5.
Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 species, representing approximately two thirds of the bacterial diversity within the sampled bladders, including Proteobacteria, Actinobacteria, and Firmicutes. Detailed genomic and functional comparison of the bladder microbiota to the gastrointestinal and vaginal microbiotas demonstrates similar vaginal and bladder microbiota, with functional capacities that are distinct from those observed in the gastrointestinal microbiota. Whole-genome phylogenetic analysis of bacterial strains isolated from the vagina and bladder in the same women identifies highly similar Escherichia coli, Streptococcus anginosus, Lactobacillus iners, and Lactobacillus crispatus, suggesting an interlinked female urogenital microbiota that is not only limited to pathogens but is also characteristic of health-associated commensals.
宏基因组分析表明，女性膀胱中存在本土微生物群。然而，可用于功能和实验分析的具有测序基因组的培养参考菌株很少。在这里，我们从 77 名女性的导尿尿液中分离并测序了 149 株细菌。该培养物集合涵盖了 78 个物种，代表了所采样膀胱内细菌多样性的大约三分之二，包括变形菌门、放线菌门和厚壁菌门。对膀胱微生物群与胃肠道和阴道微生物群的详细基因组和功能比较表明，阴道和膀胱微生物群相似，其功能能力与胃肠道微生物群观察到的不同。从同一女性阴道和膀胱中分离的细菌菌株的全基因组系统发育分析确定了高度相似的大肠杆菌、咽峡炎链球菌、惰性乳杆菌和卷曲乳杆菌，表明女性泌尿生殖道微生物群不仅限于病原体，而且还与健康相关的共生菌有关。
