Jain Raka, Quraishi Rizwana, Ambekar Atul, Verma Arpita, Gupta Pratibha
National Drug Dependence Treatment Centre, All India Institute of Medical Sciences, New Delhi, India.
Indian J Pharmacol. 2017 Nov-Dec;49(6):465-469. doi: 10.4103/ijp.IJP_578_16.
Benzodiazepines (BZD) are widely prescribed to substance users. However, the nonmedical use of prescription BZD often leads to abuse and dependence. Therefore, it is important to detect BZD among substance users seeking treatment. The aim of the present study was to develop an efficient method for testing BZD on dried urine spot (DUS) and evaluating its clinical applicability.
This involved optimization of conditions for the detection, recovery, and stability of BZD from dried urine, spotted on filter paper. Enzyme linked immuno-sorbent assay was used for screening whereas confirmation was done by gas chromatography. For clinical applicability, urine samples of BZD users were tested.
The recovery was found to be 99.7% in de-ionized water from 20 μl spotted urine samples. Limit of detection, inter-day and intra-day CV were found to be 100 ng/ml, 4.22% and 3.83%, respectively. BZD were found stable in DUS for 3 weeks at room temperature, and for 3 months at 4°C and -20°C. All the urine samples of benzodiazepine users were found positive by conventional method as well as the DUS method.
DUS method proved to be efficient for BZD testing with advantages of ease of collection, transportation, minimal invasiveness and small sample volume. It offers a useful alternative for BZD testing especially in developing countries where logistics of sample collection and transportation could be an important concern.
苯二氮䓬类药物（BZD）被广泛开给药物使用者。然而，处方BZD的非医疗使用常常导致滥用和依赖。因此，在寻求治疗的药物使用者中检测BZD很重要。本研究的目的是开发一种在干尿斑（DUS）上检测BZD并评估其临床适用性的有效方法。
这涉及优化从滤纸上斑点状干尿中检测、回收和稳定BZD的条件。采用酶联免疫吸附测定法进行筛查，而通过气相色谱法进行确证。为评估临床适用性，对BZD使用者的尿液样本进行检测。
在20 μl斑点状尿液样本的去离子水中，回收率为99.7%。检测限、日间和日内变异系数分别为100 ng/ml、4.22%和3.83%。BZD在室温下于DUS中稳定3周，在4°C和 -20°C下稳定3个月。所有苯二氮䓬类药物使用者的尿液样本通过传统方法和DUS方法均检测为阳性。
DUS方法被证明对BZD检测有效，具有采集、运输方便，侵入性最小和样本量小的优点。它为BZD检测提供了一种有用的替代方法，特别是在样本采集和运输的后勤可能是一个重要问题的发展中国家。
