Zhang Wen-Ying, Jin Zhen-Dong, Liu Feng, Yuan Hai-Hua, Jiang Bin
Department of Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Department of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai, China.
Gastroenterol Res Pract. 2018 Feb 21;2018:7149565. doi: 10.1155/2018/7149565. eCollection 2018.
Pancreatic cancer is a lethal disease and usually is diagnosed at advanced stages of disease. This study assessed the effects of intratumoral ethanol injection using an endoscopic ultrasound (EUS) probe on the control of pancreatic cancer in a mouse orthotopic xenograft model.
The subcutaneous and orthotopic human pancreatic cancer cell mouse xenograft models were established. Different concentrations of ethanol (0-95%) were injected into subcutaneous xenograft tumors. In the orthotopic tumor model, ethanol was injected into the tumor lesions under the guidance of a high-frequency EUS probe. Tumor volume, relative tumor volume (RTV), and histopathology were evaluated. The serum amylase level was analyzed at baseline and 24 h after treatment in the orthotopic tumor model.
Injection of 40-95% ethanol induced tumor necrosis in the subcutaneous tumor model, while there was no statistical difference between the RTVs of the two groups ( = 0.81). In the orthotopic tumor model, the RTV of the 80% ethanol treatment group was less than that of the saline injection group ( < 0.01); and histologically, there was a large area of necrosis observed in the 80% ethanol group. The serum amylase level was slightly elevated at 24 h after injection and returned to the baseline level at 7 days.
Injection of 80% ethanol into xenograft tumor lesions of orthotopic pancreatic cancer resulted in tumor necrosis, and the procedure was safe and effective. Future studies will further confirm its antitumor activity as well as assess its safety and feasibility.
胰腺癌是一种致命疾病，通常在疾病晚期才被诊断出来。本研究在小鼠原位异种移植模型中评估了使用内镜超声（EUS）探头进行瘤内乙醇注射对胰腺癌控制的效果。
建立皮下和原位人胰腺癌细胞小鼠异种移植模型。将不同浓度（0 - 95%）的乙醇注射到皮下异种移植瘤中。在原位肿瘤模型中，在高频EUS探头引导下将乙醇注射到肿瘤病灶内。评估肿瘤体积、相对肿瘤体积（RTV）和组织病理学。在原位肿瘤模型中，于基线和治疗后24小时分析血清淀粉酶水平。
在皮下肿瘤模型中，注射40 - 95%乙醇可诱导肿瘤坏死，但两组的RTV之间无统计学差异（ = 0.81）。在原位肿瘤模型中，80%乙醇治疗组的RTV小于生理盐水注射组（ < 0.01）；组织学检查显示，80%乙醇组有大面积坏死。注射后24小时血清淀粉酶水平略有升高，7天时恢复至基线水平。
向原位胰腺癌异种移植瘤病灶内注射80%乙醇可导致肿瘤坏死，且该操作安全有效。未来研究将进一步证实其抗肿瘤活性，并评估其安全性和可行性。
