Poon K W C, Brideau C, Klaver R, Schenk G J, Geurts J J, Stys P K
Hotchkiss Brain Institute , Cumming School of Medicine , University of Calgary , Canada . Email:
Department of Anatomy and Neurosciences , Vrije University Medical Center , Amsterdam , The Netherlands . Email:
Chem Sci. 2018 Jan 2;9(6):1586-1595. doi: 10.1039/c7sc03992a. eCollection 2018 Feb 14.
Multiple sclerosis (MS) exhibits demyelination, inflammatory infiltration, axonal degeneration, and gliosis, affecting widespread regions of the central nervous system (CNS). While white matter MS lesions have been well characterized pathologically, evidence indicates that the MS brain may be globally altered, with subtle abnormalities found in grossly normal appearing white matter (NAWM). These subtle changes are difficult to investigate by common methods such as histochemical stains and conventional magnetic resonance imaging. Thus, the prototypical inflammatory lesion likely represents the most obvious manifestation of a more widespread involvement of the CNS. We describe the application of spectral coherent anti-Stokes Raman Scattering (sCARS) microscopy to study such changes in chronic MS tissue particularly in NAWM. Subtle changes in myelin lipid biochemical signatures and intra-molecular disorder of fatty acid acyl chains of otherwise normal-appearing myelin were detected, supporting the notion that the biochemical involvement of the MS brain is far more extensive than conventional methods would suggest.
多发性硬化症（MS）表现为脱髓鞘、炎症浸润、轴突变性和胶质增生，影响中枢神经系统（CNS）的广泛区域。虽然白质MS病变在病理上已得到充分表征，但有证据表明，MS脑可能整体发生改变，在外观大体正常的白质（NAWM）中发现了细微异常。这些细微变化很难通过组织化学染色和传统磁共振成像等常用方法进行研究。因此，典型的炎性病变可能是CNS更广泛受累的最明显表现。我们描述了光谱相干反斯托克斯拉曼散射（sCARS）显微镜在研究慢性MS组织尤其是NAWM中的此类变化方面的应用。检测到外观正常的髓鞘的髓磷脂脂质生化特征和脂肪酸酰基链的分子内无序的细微变化，支持了MS脑的生化受累远比传统方法所显示的更为广泛的观点。
