Wang Qian, Zhao Rui, Cui Yan, Liu Cuicui, Liang Liya, Yan Shijie
Department of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China.
College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
J Anal Methods Chem. 2018 Feb 20;2018:1981436. doi: 10.1155/2018/1981436. eCollection 2018.
A simple and rapid capillary electrophoresis method with diode array detector was developed for determination of sarafloxacin and difloxacin in beef. In this study, the experimental parameters affecting the determination were systematically optimized, including wavelength, buffer system, pH and concentration, and separation temperature and voltage. Under the optimal conditions, sarafloxacin and difloxacin could be quantified within 4 min using HBO/NaBO buffer (35 mmol/L, pH 8.8) as background electrolyte, 25 kV as separation voltage, and 22°C as the column temperature. The linear range of the method was 1-20 g/mL with LOD 0.8 g/mL for sarafloxacin and 0.5-20 g/mL with LOD 0.3 g/mL for difloxacin. The RSDs for the peak area of 8 g/mL sarafloxacin were 4.8% (intraday) and 7.8% (interday), respectively. The proposed method has been applied to determine the residue of sarafloxacin and difloxacin in beef samples with the satisfactory recovery.
建立了一种采用二极管阵列检测器的简单快速毛细管电泳法，用于测定牛肉中的沙拉沙星和二氟沙星。在本研究中，系统优化了影响测定的实验参数，包括波长、缓冲体系、pH值和浓度，以及分离温度和电压。在最佳条件下，以HBO/NaBO缓冲液（35 mmol/L，pH 8.8）作为背景电解质，25 kV作为分离电压，22°C作为柱温，沙拉沙星和二氟沙星可在4分钟内定量。该方法的线性范围为1-20 μg/mL，沙拉沙星的检测限为0.8 μg/mL，二氟沙星的线性范围为0.5-20 μg/mL，检测限为0.3 μg/mL。8 μg/mL沙拉沙星峰面积的日内和日间相对标准偏差分别为4.8%和7.8%。该方法已应用于牛肉样品中沙拉沙星和二氟沙星残留的测定，回收率令人满意。
