Zhang Xiaowei, Zhang Zhaoxiang
School of Science, Qingdao Technological University, Qingdao 266033, China.
J Chromatogr Sci. 2013 Feb;51(2):107-11. doi: 10.1093/chromsci/bms112. Epub 2012 Jul 10.
Neurotoxic shellfish poisoning is a significant food-borne disease caused by potent cyclic polyether toxins (brevetoxins) that accumulate in the flesh of shellfish. Here we report a new procedure for brevetoxin analysis by capillary electrophoresis-based immunoassay (CE-IA) with electrochemical (EC) detection. In this method, after the competitive immunoreaction in liquid phase, the horseradish peroxidase (HRP)-labeled antigen and the bound enzyme-labeled complex were separated and then the system of HRP catalyzing H(2)O(2)-o-aminophenol reaction was adopted. The limits of detection (signal-to-noise = 3) was determined to be 0.1 ng/mL. Intra-day relative standard deviations (RSD, n = 5) for migration time and peak area were 3.6 and 4.5%, respectively. Inter-day RSD was 6.9 and 7.8% for migration time and peak area, respectively. The CE-IA with EC detection provides a sensitive analytical approach, not previously available, for the determination of brevetoxin-B in shellfish samples.
神经性贝类中毒是一种由积聚在贝类体内的强效环状聚醚毒素（短裸甲藻毒素）引起的重要食源性疾病。在此，我们报告一种基于毛细管电泳免疫分析（CE-IA）并结合电化学（EC）检测的短裸甲藻毒素分析新方法。在该方法中，液相竞争免疫反应后，分离辣根过氧化物酶（HRP）标记的抗原和结合的酶标记复合物，然后采用HRP催化H₂O₂-邻氨基酚反应体系。检测限（信噪比=3）确定为0.1 ng/mL。迁移时间和峰面积的日内相对标准偏差（RSD，n = 5）分别为3.6%和4.5%。迁移时间和峰面积的日间RSD分别为6.9%和7.8%。基于EC检测的CE-IA为测定贝类样品中的短裸甲藻毒素-B提供了一种前所未有的灵敏分析方法。
