Talukder Muhammad Anisuzzaman, Menyuk Curtis R, Kostov Yordan
Department of Electrical and Electronic Engineering, Bangladesh University of Engineering and Technology, Dhaka 1205, Bangladesh.
School of Electronic and Electrical Engineering, University of Leeds, Leeds LS2 9JT, United Kingdom.
Biomed Opt Express. 2018 Mar 29;9(4):1977-1991. doi: 10.1364/BOE.9.001977. eCollection 2018 Apr 1.
Distinguishing between whole cells and cell debris is important in microscopy, e.g., in screening of pulmonary patients for infectious tuberculosis. We propose and theoretically demonstrate that whole cells and cell debris can be distinguished from the far-field pattern of surface plasmon coupled emission (SPCE) of a fluorescently-labeled sample placed on a thin metal layer. If fluorescently-labeled whole cells are placed on the metal film, SPCE takes place simultaneously at two or more different angles and creates two or more distinct rings in the far field. By contrast, if fluorescently-labeled cell debris are placed on the metal film, SPCE takes place at only one angle and creates one ring in the far-field. We find that the angular separation of the far-field rings is sufficiently distinct to use the presence of one or more rings to distinguish between whole cells and cell debris. The proposed technique has the potential for detection without the use of a microscope.
区分完整细胞和细胞碎片在显微镜检查中很重要，例如在筛查肺部患者是否感染肺结核时。我们提出并从理论上证明，可以根据放置在薄金属层上的荧光标记样品的表面等离子体耦合发射（SPCE）的远场模式来区分完整细胞和细胞碎片。如果将荧光标记的完整细胞放置在金属膜上，SPCE会在两个或更多不同角度同时发生，并在远场中产生两个或更多个不同的环。相比之下，如果将荧光标记的细胞碎片放置在金属膜上，SPCE仅在一个角度发生，并在远场中产生一个环。我们发现远场环的角间距足够明显，可以利用一个或多个环的存在来区分完整细胞和细胞碎片。所提出的技术具有无需使用显微镜即可进行检测的潜力。
