Three-dimensional imaging of biological cells using surface plasmon coupled emission.

SIGNIFICANCE

Biological cell imaging has become one of the most crucial research interests because of its applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells is critically challenging and often involves prohibitively expensive and complex equipment. Therefore, a low-cost imaging technique with a simpler optical arrangement is immensely needed.

AIM

The proposed approach will provide an accurate cell image at a low cost without needing any microscope or extensive processing of the collected data, often used in conventional imaging techniques.

APPROACH

We propose that patterns of surface plasmon coupled emission (SPCE) features from a fluorescently labeled biological cell can be used to image the cell. An imaging methodology has been developed and theoretically demonstrated to create 3D images of cells from the detected SPCE patterns. The 3D images created from the different SPCE properties at the far-field closely match the actual cell structures.

RESULTS

The developed technique has been applied to different regular and irregular cell shapes. In each case, the calculated root-mean-square error (RMSE) of the created images from the cell structures remains within a few percentages. Our work recreates the base of a circular-shaped cell with an RMSE of ≲1.4 % . In addition, the images of irregular-shaped cell bases have an RMSE of ≲2.8 % . Finally, we obtained a 3D image with an RMSE of ≲6.5 % for a random cellular structure.

CONCLUSIONS

Despite being in its initial stage of development, the proposed technique shows promising results considering its simplicity and the nominal cost it would require.