Department of Ophthalmology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Department of Ophthalmology, Shanghai Zhabei District Central Hospital, Shanghai 200003, China.
Biomed Res Int. 2018 Feb 20;2018:5961974. doi: 10.1155/2018/5961974. eCollection 2018.
Thyroid associated ophthalmopathy (TAO) is an autoimmune disease, which involves inflammation and tissue remodeling. Pentraxin-3 (PTX3) is a component of innate immune system and recently implicated in autoimmunity. This observation may indicate that PTX3 participates in the inflammatory process of TAO.
All studies were performed on TAO patients and healthy controls (45: 28 in total). RNA-seq was used to detect differential gene expression of orbital adipose-connective tissue. Quantitative PCR was performed to verify the results. PTX3 protein in orbital adipose-connective tissues was visualized by immunohistochemistry (IHC). PTX3 concentration in serum was determined by enzyme-linked immunosorbent assay (ELISA).
RNA-seq showed 1.86-log2FC higher PTX3 expression in the orbital adipose-connective tissues from TAO group than controls (FDR = 0.0059). qPCR confirmed the difference (5.59-fold increase, = 0.0012). The presence of PTX3 protein was demonstrated. Orbital adipose tissue from healthy controls showed weak staining for PTX3 while tissue from TAO group was strongly positive. Serum PTX3 concentration was significantly elevated in patients when compared to the control group (1.9-fold increase; < 0.0001).
Patients with TAO showed increased presence of PTX3 in orbital tissue and serum, which may suggest a potential relationship of PTX3 and TAO.
甲状腺相关眼病（TAO）是一种自身免疫性疾病，涉及炎症和组织重塑。五聚素 3（PTX3）是先天免疫系统的组成部分，最近与自身免疫有关。这一观察结果可能表明 PTX3 参与了 TAO 的炎症过程。
所有研究均在 TAO 患者和健康对照者（共 45 例：28 例）中进行。使用 RNA 测序检测眼眶脂肪-结缔组织中的差异基因表达。进行定量 PCR 以验证结果。通过免疫组织化学（IHC）显示眼眶脂肪-结缔组织中的 PTX3 蛋白。通过酶联免疫吸附测定（ELISA）测定血清中的 PTX3 浓度。
RNA 测序显示 TAO 组眼眶脂肪-结缔组织中的 PTX3 表达比对照组高 1.86-log2FC（FDR = 0.0059）。qPCR 证实了这一差异（增加 5.59 倍， = 0.0012）。存在 PTX3 蛋白。健康对照组的眼眶脂肪组织对 PTX3 的染色较弱，而 TAO 组的组织则呈强阳性。与对照组相比，患者的血清 PTX3 浓度显著升高（增加 1.9 倍；<0.0001）。
TAO 患者眼眶组织和血清中 PTX3 的存在增加，这可能表明 PTX3 与 TAO 之间存在潜在关系。
