Department of Biosystems Engineering, Auburn University, Auburn, AL, 36849, USA.
The Alabama Center for Paper and Bioresource Engineering, Auburn University, Auburn, AL, 36849, USA.
Appl Microbiol Biotechnol. 2018 Jun;102(11):4765-4772. doi: 10.1007/s00253-018-8985-6. Epub 2018 Apr 19.
The aim of this work was to study the solid waste (manure) produced by catfish as a potential feedstock for the production of lactic acid (LA) via fermentation. The solid waste contains high levels of both carbohydrates and nutrients that are sufficient for LA bacteria. Simultaneous saccharification and co-fermentation (SSCF) was applied using enzyme and Lactobacillus pentosus, and different loadings of enzyme and solid waste were tested. Results showed LA concentrations of 35.7 g/L were obtained at 15% solids content of catfish waste. Because of the high nutrient content in the fish waste, it could also be used as supplementary substrate for nitrogen and carbon sources with other lignocellulosic materials. A combined feedstock of catfish waste and paper mill sludge was tested, increasing the final LA concentration to 43.1 g/L at 12% solids loading. The catfish waste was shown to be a potential feedstock to provide both carbon and nutrients for LA production, suggesting its use as a sole substrate or in combination with other lignocellulosic materials.
本工作旨在研究鲶鱼产生的固体废物（粪便），将其作为通过发酵生产乳酸（LA）的潜在原料。固体废物中含有大量的碳水化合物和营养物质，足以满足 LA 细菌的需求。使用酶和戊糖片球菌进行同步糖化和共发酵（SSCF），并测试了不同的酶和固体废物负荷。结果表明，在鲶鱼废物的固体含量为 15%时，可获得 35.7g/L 的 LA 浓度。由于鱼废物中含有丰富的营养物质，它也可以作为补充氮源和碳源的底物，与其他木质纤维素材料一起使用。还测试了鲶鱼废物和造纸厂污泥的组合原料，在 12%的固体负荷下，最终 LA 浓度增加到 43.1g/L。研究表明，鲶鱼废物是一种有潜力的原料，可以为 LA 生产提供碳源和营养物质，这表明它可以单独用作底物或与其他木质纤维素材料结合使用。
