Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
J Biomed Mater Res B Appl Biomater. 2019 Apr;107(3):511-520. doi: 10.1002/jbm.b.34141. Epub 2018 Apr 19.
Nanofibrous poly(l-lactic acid) (PLLA) microspheres are extensively studied to be used as cell carriers in the field of tissue engineering because the unique structure can promote cell proliferation and migration. But as injectable scaffold materials, PLLA microspheres easily run off to the soft tissue space because of the lack of cohesive force. It will affect the treatment efficiency and even cause additional inflammatory response. In order to overcome this disadvantage, superparamagnetic γ-Fe O nanoparticles assisted with oxidative polymerization of dopamine were used for surface modification of PLLA microspheres in this study. The results showed that this surface modification had no obvious cytotoxicity, and the modified microspheres possessed the ability to carry seed cells to controllably move to the defect sites with the guidance of magnetic field, which may be able to increase the repair efficiency. Moreover, the characteristic nanofibrous structure was not destroyed after modification, which was able to promote biological activity of cells. This work provides a novel way to produce superparamagnetic nanofibrous microspheres designed for cell microcarriers. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 511-520, 2019.
纳米纤维聚（L-丙交酯）（PLLA）微球被广泛研究用作组织工程领域的细胞载体，因为其独特的结构可以促进细胞增殖和迁移。但是，作为可注射支架材料，由于缺乏内聚力，PLLA 微球很容易跑到软组织间隙中，这会影响治疗效果，甚至引起额外的炎症反应。为了克服这一缺点，本研究采用超顺磁 γ-FeO 纳米粒子辅助多巴胺的氧化聚合对 PLLA 微球进行表面改性。结果表明，这种表面改性没有明显的细胞毒性，并且改性后的微球具有携带种子细胞的能力，可以在磁场的引导下可控地移动到缺陷部位，这可能能够提高修复效率。此外，改性后并未破坏其特有的纳米纤维结构，这能够促进细胞的生物活性。这项工作为设计用于细胞微载体的超顺磁纳米纤维微球的生产提供了一种新方法。©2018 年 Wiley 期刊出版公司。J 生物医学材料研究杂志 B：应用生物材料，2018 年。©2018 年 Wiley 期刊出版公司。J 生物医学材料研究杂志 B：应用生物材料 107B:511-520,2019 年。
