Li Bo, Yang Haocheng, Cheng Kaiyuan, Song Hongli, Zou Jie, Li Chenchen, Xiao Wenqian, Liu Zhongning, Liao Xiaoling
Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing Engineering Laboratory of Nano/Micro Biomedical Detection Technology, Chongqing University of Science and Technology, Chongqing 401331, China.
Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China.
Colloids Surf B Biointerfaces. 2023 Mar;223:113175. doi: 10.1016/j.colsurfb.2023.113175. Epub 2023 Jan 31.
To avoid infection and other risks caused by large open-surgery incisions using scaffold transplants, it is very important to study injectable microcarrier-loaded cells for targeted therapy and tissue regeneration. In this study, on the one hand, to simulate the hierarchical structure of the extracellular matrix and carry cells, poly(L-lactic acid) (PLLA) nanofibrous microspheres (large microspheres) were initially fabricated as cell carriers. On the other hand, to precisely deliver cells through a magnetic field and promote stem cell differentiation, drug-loaded mesoporous FeO@SiO microspheres (small microspheres) were prepared and coated on the surface PLLA nanofibrous microspheres. The coating conditions were systematically studied and optimized. The results showed that planetary-satellite-like cell carriers were successfully prepared and the carriers were capable of freely translocating under the influence of a magnetic field. It has been demonstrated in vitro experiments that the carriers are biocompatible and are capable of acting as drug carriers. Specifically, they were able to load and release cells in response to magnetic fields. In vivo experiments indicated that the carriers could successfully load and release GFP-labelled cells in nude mice. The study presented in this paper provides a versatile and promising platform for the cell-based therapy in tissue engineering and regenerative medicine.
为避免使用支架移植的大型开放手术切口所带来的感染及其他风险，研究用于靶向治疗和组织再生的可注射微载体负载细胞具有重要意义。在本研究中，一方面，为模拟细胞外基质的层级结构并携带细胞，首先制备了聚（L-乳酸）（PLLA）纳米纤维微球（大微球）作为细胞载体。另一方面，为通过磁场精确递送细胞并促进干细胞分化，制备了载药介孔FeO@SiO微球（小微球）并包覆在表面PLLA纳米纤维微球上。对包覆条件进行了系统研究和优化。结果表明成功制备了行星-卫星状细胞载体，且该载体能够在磁场影响下自由移动。体外实验证明该载体具有生物相容性且能够作为药物载体。具体而言，它们能够响应磁场加载和释放细胞。体内实验表明该载体能够在裸鼠体内成功加载和释放绿色荧光蛋白标记的细胞。本文所呈现的研究为组织工程和再生医学中基于细胞的治疗提供了一个通用且有前景的平台。
