Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR, USA.
J Appl Microbiol. 2018 Aug;125(2):313-327. doi: 10.1111/jam.13888. Epub 2018 Jun 26.
Salmonella remains a prominent cause of foodborne illnesses and can originate from a wide range of food products. Given the continued presence of pathogenic Salmonella in food production systems, there is a consistent need to improve identification and detection methods that can identify this pathogen at all stages in food systems. Methods for subtyping have evolved over the years, and the introduction of whole genome sequencing and advancements in PCR technologies have greatly improved the resolution for differentiating strains within a particular serovar. This, in turn, has led to the continued improvement in Salmonella detection technologies for utilization in food production systems. In this review, the focus will be on recent advancements in these technologies, as well as potential issues associated with the application of these tools in food production. In addition, the recent and emerging research developments on Salmonella detection and identification methodologies and their potential application in food production systems will be discussed.
沙门氏菌仍然是食源性疾病的主要病原体,可能源自多种食品。鉴于食源性生产系统中持续存在致病性沙门氏菌,因此一直需要改进能够在食品系统的各个阶段识别这种病原体的鉴定和检测方法。多年来,分型方法不断发展,全基因组测序和 PCR 技术的进步极大地提高了在特定血清型内区分菌株的分辨率。这反过来又导致了用于食品生产系统的沙门氏菌检测技术的持续改进。在本次综述中,重点将放在这些技术的最新进展上,以及在食品生产中应用这些工具相关的潜在问题上。此外,还将讨论沙门氏菌检测和鉴定方法的最新和新兴研究进展及其在食品生产系统中的潜在应用。