College of Forestry, Northwest A&F University, Yangling, China.
College of Biological Resource and Food Engineering, Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan, China.
J Appl Microbiol. 2018 Aug;125(2):554-563. doi: 10.1111/jam.13883. Epub 2018 May 31.
This study investigates pinewood nematode's impacts on root mycoflora of Pinus tabulaeformis.
The biomass, colonization rate, community structure and diversity of root-associated fungi were investigated in pinewood nematode-infected and nematode-noninfected P. tabulaeformis. The results indicated that the roots of P. tabulaeformis were colonized highly by root-associated fungi, mainly ectomycorrhizal fungi (ECMF) and dark septate endophytes. Infection of pinewood nematode was associated with a significant (P < 0·05) decrease in root colonization rates by ECMF, dark septate endophytes and total hyphae, as well as in fungal biomass in the roots. Illumina MiSeq sequences of tagged amplicons of 18S rDNA region revealed Basidiomycota (65·70%) and Ascomycota (34·14%) as the dominant root-associated fungi in roots of P. tabulaeformis. Among the detected operational taxonomic units (OTUs), ECMF and dark septate endophytes exhibited a higher relative abundance in trees infected by pinewood nematode compared with noninfected ones.
The infection of pinewood nematode altered the composition and OTU abundance of root-associated fungi community in P. tabulaeformis roots with a decrease in the biomass, species richness and diversity of root-associated fungi, as well as in the colonization rates and abundance of ECMF and dark septate endophytes.
This study is an important contribution for better understanding the interaction between pine wilt disease and root-associated fungi.
本研究调查了松材线虫对油松根际真菌区系的影响。
调查了感染和未感染松材线虫的油松根际真菌的生物量、定殖率、群落结构和多样性。结果表明，油松根被根际真菌高度定殖，主要是外生菌根真菌（ECMF）和暗隔内生真菌。松材线虫感染与 ECMF、暗隔内生真菌和总菌丝的根定殖率以及根中真菌生物量的显著（P<0.05）下降有关。18S rDNA 区标记扩增子的 Illumina MiSeq 序列显示，担子菌门（65.70%）和子囊菌门（34.14%）是油松根际的主要根际真菌。在所检测的操作分类单元（OTUs）中，与未感染的油松相比，感染松材线虫的油松中 ECMF 和暗隔内生真菌的相对丰度更高。
松材线虫的感染改变了油松根际真菌群落的组成和 OTU 丰度，导致根际真菌的生物量、物种丰富度和多样性下降，以及 ECMF 和暗隔内生真菌的定殖率和丰度下降。
本研究为更好地理解松材线虫病与根际真菌的相互作用提供了重要贡献。
