Wagg Cameron, Pautler Michael, Massicotte Hugues B, Peterson R Larry
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.
Mycorrhiza. 2008 Feb;18(2):103-10. doi: 10.1007/s00572-007-0157-y. Epub 2007 Dec 19.
Although roots of species in the Pinaceae are usually colonized by ectomycorrhizal (EM) fungi, there are increasing reports of the presence of arbuscular mycorrhizal (AM) and dark septate endophytic (DSE) fungi in these species. The objective of this study was to determine the colonization patterns in seedlings of three Pinus (pine) species (Pinus banksiana, Pinus strobus, Pinus contorta) and Picea glauca x Picea engelmannii (hybrid spruce) grown in soil collected from a disturbed forest site. Seedlings of all three pine species and hybrid spruce became colonized by EM, AM, and DSE fungi. The dominant EM morphotype belonged to the E-strain category; limited colonization by a Tuber sp. was found on roots of Pinus strobus and an unknown morphotype (cf. Suillus-Rhizopogon group) with thick, cottony white mycelium was present on short roots of all species. The three fungal categories tended to occupy different niches in a single root system. No correlation was found between the percent root colonized by EM and percent colonization by either AM or DSE, although there was a positive correlation between percent root length colonized by AM and DSE. Hyphae and vesicles were the only AM intracellular structures found in roots of all species; arbuscules were not observed in any roots.
尽管松科植物的根系通常会被外生菌根（EM）真菌定殖，但越来越多的报道表明，这些物种中也存在丛枝菌根（AM）和深色有隔内生菌（DSE）真菌。本研究的目的是确定在从一个受干扰的森林地点采集的土壤中生长的三种松树（班克松、美国白松、扭叶松）和白云杉×恩氏云杉（杂交云杉）幼苗的定殖模式。所有三种松树和杂交云杉的幼苗都被EM、AM和DSE真菌定殖。主要的EM形态型属于E菌株类别；在北美乔松的根上发现了少量块菌属的定殖，并且在所有物种的短根上都存在一种未知形态型（类似于乳牛肝菌-须腹菌属组），其具有浓密的棉絮状白色菌丝体。这三类真菌在单个根系中倾向于占据不同的生态位。尽管AM和DSE定殖的根长度百分比之间存在正相关，但未发现EM定殖的根百分比与AM或DSE定殖百分比之间存在相关性。菌丝和泡囊是在所有物种的根中发现的仅有的AM细胞内结构；在任何根中均未观察到丛枝。
