基于psbA-trnH条形码对穿龙薯蓣及其组的鉴定

[Identification of Dioscorea nipponica and its sects based on psbA-trnH barcode].

作者信息

Zhao Rong, Shao Fei, Yin Hai-Bo, Kang Ting-Guo

机构信息

Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2018 Mar;43(5):938-944. doi: 10.19540/j.cnki.cjcmm.20180109.007.

DOI:10.19540/j.cnki.cjcmm.20180109.007

PMID:29676091

Abstract

A rapid and accurate method was established by psbA-trnH sequence to distinguish Dioscorea nipponica from other species belonging to the same genus.In this study, we collected 144 samples of D. nipponica from 17 different producing areas in China, the psbA-trnH sequenced of 23 nucleotide sequences were downloaded from GenBank, the sequences from Dioscorea genus. DNAMAN 8.0 software was used to show splicing, MEGA 6.0 software was applied for sequence analysis and comparison. The results showed that the genetic relationship between D. nipponica and D. subcalva was the closest, and the genetic relationship with D. zingiberensis was the furthest. It is indicated that the psbA-trnH sequence as a DNA barcode can effectively distinguish between D. nipponica and other plants in the genus of Dioscorea.

摘要

建立了一种基于psbA-trnH序列的快速准确区分穿龙薯蓣与同属其他物种的方法。本研究从中国17个不同产地收集了144份穿龙薯蓣样本，从GenBank下载了23条来自薯蓣属的核苷酸序列的psbA-trnH序列。使用DNAMAN 8.0软件进行拼接展示，应用MEGA 6.0软件进行序列分析和比较。结果表明，穿龙薯蓣与盾叶薯蓣的亲缘关系最近，与黄独的亲缘关系最远。表明psbA-trnH序列作为DNA条形码能够有效区分穿龙薯蓣与薯蓣属其他植物。