a College of Bioscience & Biotechnology, Hunan Agricultural University, Changsha 410128, China.
b Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
Genome. 2018 Jan;61(1):15-20. doi: 10.1139/gen-2017-0115. Epub 2017 Sep 29.
Physalis L., an important genus of the family Solanaceae, includes many commercially important edible and medicinal species. Traditionally, species identification is based on morphological traits; however, the highly similar morphological traits among species of Physalis make this approach difficult. In this study, we evaluated the feasibility of using a popular DNA barcode, the chloroplast psbA-trnH intergenic region, in the identification of species of Physalis. Thirty-six psbA-trnH regions of species of Physalis and of the closely related plant Nicandra physalodes were analyzed. The success rates of PCR amplification and sequencing of the psbA-trnH region were 100%. MEGA V6.0 was utilized to align the psbA-trnH sequences and to compute genetic distances. The results show an apparent barcoding gap between intra- and interspecific variations. Results of both BLAST1 and nearest-distance methods prove that the psbA-trnH regions can be used to identify all species examined in the present study. In addition, phylogenetic analysis using psbA-trnH data revealed a distinct boundary between species. It also confirmed the relationship between species of Physalis and closely related species, as established by previous studies. In conclusion, the psbA-trnH intergenic region can be used as an efficient DNA barcode for the identification of species of Physalis.
落葵科（Solanaceae）的重要属——酸浆属（Physalis），包括许多具有商业重要性的食用和药用物种。传统上，物种鉴定基于形态特征；然而，酸浆属物种之间高度相似的形态特征使得这种方法变得困难。在这项研究中，我们评估了使用流行的 DNA 条码——叶绿体 psbA-trnH 基因间隔区，来鉴定酸浆属物种的可行性。分析了 36 种酸浆属和近缘植物假酸浆（Nicandra physalodes）的 psbA-trnH 区。psbA-trnH 区的 PCR 扩增和测序成功率均为 100%。MEGA V6.0 用于 psbA-trnH 序列的比对和遗传距离的计算。结果显示，种内和种间变异之间存在明显的条形码间隙。BLAST1 和最近距离方法的结果均证明 psbA-trnH 区可用于鉴定本研究中检查的所有物种。此外，基于 psbA-trnH 数据的系统发育分析显示，物种之间存在明显的界限。它还证实了酸浆属物种与先前研究确定的近缘物种之间的关系。总之，psbA-trnH 基因间隔区可作为鉴定酸浆属物种的有效 DNA 条码。
