Department of Medicine, University of British Columbia Vancouver, BC, Canada.
BC Children's Hospital Research Institute, Vancouver, BC, Canada.
Eur J Immunol. 2018 Aug;48(8):1415-1419. doi: 10.1002/eji.201747407. Epub 2018 May 16.
We optimized a method to detect FOXP3 by mass cytometry and compared the resulting data to conventional flow cytometry. We also demonstrated the utility of the protocol to profile antigen-specific Tregs from whole blood, or Tregs from tissues such as cord blood, thymus and synovial fluid.
我们优化了一种通过质谱流式细胞术检测 FOXP3 的方法,并将得到的数据与传统流式细胞术进行了比较。我们还展示了该方案从全血中分析抗原特异性 Tregs 或从脐带血、胸腺和滑液等组织中分析 Tregs 的实用性。