Ren Hui, Zhang Jiabin, Wang Hongbo, Zhou Shaotang
From the Center of Hepatobiliary Surgery and Liver Transplantation, 302 Hospital, Beijing, China.
Exp Clin Transplant. 2018 Oct;16(5):588-591. doi: 10.6002/ect.2017.0136. Epub 2018 Apr 20.
Our objective was to investigate the techniques of vessel suturing in the abdominal mouse cardiac transplant model and animal outcomes.
Our mouse group included 92 female inbred mice, in which 46 abdominal mouse heart transplants were performed. During transplant, the openings for the aorta and the inferior vena cava were not parallel. With everted anastomosis, the ascending aortae and the pulmonary arteries of donors were connected to the abdominal aortae and interior vena cavae of recipients, respectively, with 11-0 Prolene suture. We recorded complications and animal survival.
The first 2 mice that underwent the procedure died of blood loss on day 1. However, the remaining 44 mouse recipients were alive for greater than 14 days, resulting in 14-day survival rate of 95.65%. Range of time to complete the whole procedure, including learning curve, was 47 to 72 minutes. At histology, implanted hearts from recipients with long-term survival appeared normal.
The procedure for everted suturing in the abdominal mouse heart transplant model was easy to perform and had a high success rate.
我们的目的是研究腹部小鼠心脏移植模型中的血管缝合技术及动物预后情况。
我们的小鼠组包括92只雌性近交系小鼠，其中进行了46例腹部小鼠心脏移植。在移植过程中，主动脉和下腔静脉的开口不平行。采用外翻吻合术，用11-0普理灵缝线将供体的升主动脉和肺动脉分别与受体的腹主动脉和下腔静脉相连。我们记录了并发症和动物存活情况。
最初接受该手术的2只小鼠在第1天死于失血。然而，其余44只小鼠受体存活超过14天，14天生存率为95.65%。完成整个手术过程（包括学习曲线）的时间范围为47至72分钟。组织学检查显示，长期存活受体的植入心脏外观正常。
腹部小鼠心脏移植模型中的外翻缝合手术操作简便，成功率高。
