State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China.
Int J Mol Sci. 2018 Apr 20;19(4):1249. doi: 10.3390/ijms19041249.
Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of is still unknown. We cloned an Argonaute3 gene in () with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Ago3 (BmAgo3). The suppression of by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of decreased the suppression level of by dsPxActin in cells, while overexpression of increased the RNAi efficiency. Our results suggest that play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in .
Argonaute (Ago) 蛋白家族在不同昆虫包括鳞翅目昆虫的 RNA 干扰 (RNAi) 过程中发挥着关键作用。然而，Ago 蛋白在 的 RNAi 途径中的作用尚不清楚。我们在 （）中克隆了一个 Argonaute3 基因，其完整编码序列为 2832bp。编码的蛋白含有 935 个氨基酸，预计分子量为 108.9kDa，等电点为 9.29。它包含一个 PAZ（PIWI/Argonaute/Zwile）结构域和 PIWI（P-element-induced whimpy testes）结构域。PxAgo3 被归类为 Ago 蛋白的 Piwi 亚家族，与 Ago3（BmAgo3）具有 93.0%的高度相似性。dsPxAgo3 处理 3 小时后观察到对 的抑制作用，并持续到 24 小时。在 细胞中，PxActin 的 dsRNA 下调 ，而 过表达增加了 RNAi 效率。我们的结果表明， 在 的双链 RNA (dsRNA) 调控的 RNAi 途径中发挥关键作用。
