对小菜蛾 Plutella xylostella（L.）中假定的长非编码 RNA 进行全基因组鉴定和特征分析。

Genome-wide identification and characterization of putative lncRNAs in the diamondback moth, Plutella xylostella (L.).

机构信息

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China.

出版信息

Genomics. 2018 Jan;110(1):35-42. doi: 10.1016/j.ygeno.2017.08.003. Epub 2017 Aug 5.

DOI:10.1016/j.ygeno.2017.08.003

PMID:28789862

Abstract

Long non-coding RNAs (lncRNAs) are of particular interest because of their contributions to many biological processes. Here, we present the genome-wide identification and characterization of putative lncRNAs in a global insect pest, Plutella xylostella. A total of 8096 lncRNAs were identified and classified into three groups. The average length of exons in lncRNAs was longer than that in coding genes and the GC content was lower than that in mRNAs. Most lncRNAs were flanked by canonical splice sites, similar to mRNAs. Expression profiling identified 114 differentially expressed lncRNAs during the DBM development and found that majority were temporally specific. While the biological functions of lncRNAs remain uncharacterized, many are microRNA precursors or competing endogenous RNAs involved in micro-RNA regulatory pathways. This work provides a valuable resource for further studies on molecular bases for development of DBM and lay the foundation for discovery of lncRNA functions in P. xylostella.

摘要

长非编码 RNA（lncRNAs）因其在许多生物过程中的作用而备受关注。在这里，我们展示了鳞翅目害虫小菜蛾中全基因组鉴定和特征分析的假定 lncRNAs。总共鉴定了 8096 个 lncRNAs，并将其分为三组。lncRNAs 外显子的平均长度长于编码基因，GC 含量低于 mRNAs。大多数 lncRNAs 都有规范的剪接位点，类似于 mRNAs。表达谱分析发现 114 个在 DBM 发育过程中差异表达的 lncRNAs，并且发现大多数 lncRNAs 具有时间特异性。虽然 lncRNAs 的生物学功能尚未确定，但许多是 miRNA 前体或竞争性内源 RNA，参与 miRNA 调控途径。这项工作为进一步研究 DBM 发育的分子基础提供了有价值的资源，并为小菜蛾中 lncRNA 功能的发现奠定了基础。