Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
Sci Total Environ. 2018 Sep 1;635:432-442. doi: 10.1016/j.scitotenv.2018.04.023. Epub 2018 Apr 24.
It has been stated by researchers that the antibiotic polymyxin B sulfate (POL) is a key component inducing time-dependent antagonism in freshwater luminescent bacteria, Vibrio qinghaiensis sp.-Q67, exposed in the ternary mixture system of the ionic liquids, pesticide and antibiotics. However, the previous statement is limited to ternary and quaternary mixtures without considering situations such as the binary system. In order to prove the direct inducing of antagonism by POL in a more complete and systematic way, two categories of experiments (adding POL in non-antagonistic ternary system and decomposing antagonistic ternary system with POL into the binary system) have been conducted in this paper. The results showed that quaternary mixture systems (adding POL to non-antagonism ternary mixture, up verification) exhibit antagonistic action in a majority of rays, at some point in the experiment. However, by decomposing the antagonistic ternary mixtures with POL into binary mixtures (down verification), the combined toxicities of binary mixtures at all time points in the experiment are additive. Obviously, the POL has a significant contribution to antagonism only in the ternary and quaternary mixtures, but not in the binary mixtures. We can draw a new conclusion that the antagonism of the multi-component mixtures is induced by at least three components (including POL), with complex chemical interactions. Therefore, considering POL's influence of antagonism as an example, future environmental protection practitioners and academic researchers should construct more scenarios of mixtures when assessing the influences and reactions of certain chemicals causing pollutions.
研究人员指出，抗生素硫酸多粘菌素 B（POL）是在离子液体、农药和抗生素三元混合体系中暴露的淡水发光菌青海弧菌（Vibrio qinghaiensis sp.-Q67）产生时间依赖性拮抗作用的关键组成部分。然而，此前的说法仅限于三元和四元混合物，而没有考虑二元系统等情况。为了更完整、系统地证明 POL 对拮抗作用的直接诱导作用，本文进行了两类实验（在非拮抗三元体系中添加 POL 和用 POL 将拮抗三元体系分解为二元体系）。结果表明，在大多数射线中，四元混合物体系（在非拮抗三元混合物中添加 POL，向上验证）在实验的某些点表现出拮抗作用。然而，通过用 POL 将拮抗三元混合物分解为二元混合物（向下验证），实验中所有时间点二元混合物的联合毒性都是相加的。显然，POL 仅在三元和四元混合物中对拮抗作用有显著贡献，而在二元混合物中则没有。我们可以得出一个新的结论，即多组分混合物的拮抗作用是由至少三种成分（包括 POL）引起的，它们之间存在复杂的化学相互作用。因此，以 POL 的拮抗作用为例，未来的环境保护从业者和学术研究人员在评估某些造成污染的化学物质的影响和反应时，应该构建更多的混合物场景。
