Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
Plant Physiol Biochem. 2018 Jun;127:299-309. doi: 10.1016/j.plaphy.2018.02.001. Epub 2018 Apr 4.
Common buckwheat (F. esculentum), annually herbaceous crop, is prevalent in people's daily life with the increasing development of economics. Compared with wheat, it is highly praised with high content of rutin and flavonoid. Common buckwheat is recognized as healthy food with good taste, and the product price of which such as noodles, flour, bread and so on are higher than wheat, and the seeds of which are bigger than that of tartary buckwheat, so if common buckwheat are planted more widely, people will spend less money on this healthy and delicious food. However, soil salinity has been a giant problem for agriculture production. The cultivation of salt tolerant crop varieties is an effective way to make full use of saline alkali land, and the highest salinity that the common buckwheat can sow is at 6.0%, so we chose 100 mM as the concentration of NaCl for treatment. Then we conducted transcriptome comparison between control and treatment groups. Potential regulatory genes related salt stress in common buckwheat were identified. A total of 29.36 million clean reads were produced via an illumina sequencing approach. We de novo assembled these reads into a transcriptome dataset containing 43,772 unigenes with N50 length of 1778 bp. A total of 26,672 unigenes could be found matches in public databases. GO, KEGG and Swiss-Prot classification suggested the enrichment of these unigenes in 47 sub-categories, 25 KOG and 129 pathways, respectively. We got 385 differentially expressed genes (DEGs) after comparing the transcriptome data between salt treatment and control groups. There are some genes encoded for responsing to stimulus, cell killing, metabolic process, signaling, multi-organism process, growth and cellular process might be relevant to salt stress in common buckwheat, which will provide a valuable references for the study on mechanism of salt tolerance and will be used as a genetic information for cultivating strong salt tolerant common buckwheat varieties in the future.
普通荞麦（F. esculentum）是一种一年生草本作物，随着经济的发展，在人们的日常生活中越来越普遍。与小麦相比，它以富含芦丁和类黄酮而备受赞誉。普通荞麦被认为是一种美味可口的健康食品，其面条、面粉、面包等产品价格均高于小麦，且种子比苦荞大，如果广泛种植普通荞麦，人们将在这种健康美味的食品上花费更少的钱。然而，土壤盐渍化一直是农业生产的一个大问题。培育耐盐作物品种是充分利用盐碱地的有效途径，普通荞麦可播种的最高盐度为 6.0%，因此我们选择 100 mM 的 NaCl 浓度进行处理。然后，我们对对照组和处理组进行了转录组比较。鉴定了与普通荞麦耐盐相关的潜在调控基因。通过 Illumina 测序方法共产生了 2936 万条清洁读取序列。我们将这些reads 从头组装成一个转录组数据集，包含 43772 条 unigenes，N50 长度为 1778 bp。共有 26672 条 unigenes可以在公共数据库中找到匹配。GO、KEGG 和 Swiss-Prot 分类表明，这些 unigenes在 47 个子类别、25 个 KOG 和 129 个途径中富集。在比较盐处理组和对照组的转录组数据后，我们得到了 385 个差异表达基因（DEGs）。有一些基因编码对刺激的反应、细胞杀伤、代谢过程、信号转导、多器官过程、生长和细胞过程，这些可能与普通荞麦的盐胁迫有关，这将为研究耐盐机制提供有价值的参考，并将作为未来培育强耐盐普通荞麦品种的遗传信息。
