Witherel Claire E, Graney Pamela L, Spiller Kara L
Biomaterials & Regenerative Medicine Laboratory, School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA, USA.
Methods Mol Biol. 2018;1758:161-176. doi: 10.1007/978-1-4939-7741-3_13.
Tissue engineering and regenerative medicine, facilitated by biomaterial-based therapies, hold promise for the repair, replacement, or regeneration of damaged tissue. The success or failure of all implanted biomaterials, ranging from stainless steel total joint replacements to naturally or synthetically derived skin grafts, is predominantly mediated by macrophages, the primary cell of the innate immune system. In an effort to better assess safety and efficacy of novel biomaterials, evaluating and understanding macrophage-biomaterial interactions is a necessary first step. Here, we describe the culture of macrophages on 3D biomaterials, such as decellularized human cortical bone or commercially available wound matrices, and subsequent analysis using gene expression and protein secretion to help understand how biomaterial properties may influence macrophage phenotype in vitro.
由基于生物材料的疗法推动的组织工程和再生医学，有望修复、替换或再生受损组织。从不锈钢全关节置换物到天然或合成来源的皮肤移植，所有植入生物材料的成败主要由巨噬细胞介导，巨噬细胞是先天免疫系统的主要细胞。为了更好地评估新型生物材料的安全性和有效性，评估和理解巨噬细胞与生物材料的相互作用是必要的第一步。在这里，我们描述了巨噬细胞在三维生物材料（如脱细胞人皮质骨或市售伤口基质）上的培养，以及随后使用基因表达和蛋白质分泌进行的分析，以帮助理解生物材料特性如何在体外影响巨噬细胞表型。
