Thomas Pashuck E
Department of Materials Science and Engineering, Imperial College London, London, UK.
Methods Mol Biol. 2018;1758:177-192. doi: 10.1007/978-1-4939-7741-3_14.
Peptide self-assembly is an important field in biomaterials in which short peptides are designed to aggregate into nanostructures that often form hydrogels. These peptides are typically made using solid-phase peptide synthesis (SPPS), a technique in which amino acids are added sequentially to a growing chain. This technique has been used to synthesize peptides with more than 100 amino acids. However, self-assembling peptides are designed to aggregate in solution, which often reduces coupling efficiency during synthesis and makes purification more difficult. Here, an outline of solid-phase peptide synthesis is provided, along with steps that can be used to improve the synthetic yield and purification of self-assembling peptides for regenerative medicine applications.
肽自组装是生物材料领域的一个重要方面，其中短肽被设计聚集形成通常构成水凝胶的纳米结构。这些肽通常采用固相肽合成（SPPS）方法制备，该技术是将氨基酸依次添加到不断增长的肽链上。此技术已用于合成含100多个氨基酸的肽。然而，自组装肽被设计为在溶液中聚集，这常常会降低合成过程中的偶联效率，并使纯化更加困难。本文提供了固相肽合成的概述，以及可用于提高用于再生医学应用的自组装肽的合成产率和纯化的步骤。
