基于自组装肽的水凝胶的合成与初步表征
Synthesis and primary characterization of self-assembled peptide-based hydrogels.
作者信息
Nagarkar Radhika P, Schneider Joel P
机构信息
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
出版信息
Methods Mol Biol. 2008;474:61-77. doi: 10.1007/978-1-59745-480-3_5.
Abstract
Hydrogels based on peptide self-assembly form an important class of biomaterials that find application in tissue engineering and drug delivery. It is essential to prepare peptides with high purity to achieve batch-to-batch consistency affording hydrogels with reproducible properties. Automated solid-phase peptide synthesis coupled with optimized Fmoc (9-fluorenylmethoxy-carbonyl) chemistry to obtain peptides in high yield and purity is discussed. Details of isolating a desired peptide from crude synthetic mixtures and assessment of the peptide's final purity by high-performance liquid chromatography and mass spectrometry are provided. Beyond the practical importance of synthesis and primary characterization, techniques used to investigate the properties of hydrogels are briefly discussed.
摘要
基于肽自组装的水凝胶是一类重要的生物材料，在组织工程和药物递送领域有应用。制备高纯度的肽对于实现批次间的一致性至关重要，从而能提供具有可重复性质的水凝胶。本文讨论了结合优化的Fmoc（9-芴甲氧羰基）化学方法的自动化固相肽合成，以高产率和高纯度获得肽。文中还提供了从粗合成混合物中分离所需肽以及通过高效液相色谱和质谱评估肽最终纯度的细节。除了合成和初步表征的实际重要性外，还简要讨论了用于研究水凝胶性质的技术。
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