de Oliveira Carlos Augusto, de Paula Gabardo Michelle, Guedes Roberto Maurício Carvalho, Poncet Fabrice, Blanc Dominique S, Lobato Francisco Carlos Faria, Silva Rodrigo Otávio Silveira
Veterinary School, Federal University of Minas Gerais (UFMG), Antônio Carlos Avenue, 6627, 31.270-901, Belo Horizonte, MG, Brazil.
Hospital Preventive Medicine, Lausanne University Hospital, Lausanne, Switzerland.
Anaerobe. 2018 Jun;51:61-63. doi: 10.1016/j.anaerobe.2018.04.006. Epub 2018 Apr 9.
Features of Clostridioides difficile transmission in swine and the role of rodents as C. difficile reservoir are not clear. To investigate if rodents can carry strains of C. difficile that are genetically similar to those isolated from swine, 97 fecal samples from neonatal piglets and 41 intestinal contents from rodents were collected in two farms. All samples were subjected to C. difficile culture and the presence of A/B toxins in piglet feces were accessed by commercial enzyme imunoassay (EIA). C. difficile isolates were typed by double- (DLST) and multi-locus sequence typing (MLST). C. difficile was isolated from 15.5% of piglets and 31.7% of rodents. Most isolates were identified as DLST type 4-4 and 17-5 (both are ST11), which were found in both rodents and piglets. Results of this study suggested that rodents may have a role on the transmission and spread of C. difficile strains to swine.
艰难梭菌在猪体内的传播特征以及啮齿动物作为艰难梭菌宿主的作用尚不清楚。为了研究啮齿动物是否能携带与从猪体内分离出的艰难梭菌菌株基因相似的菌株，在两个农场收集了97份新生仔猪粪便样本和41份啮齿动物肠道内容物样本。所有样本均进行艰难梭菌培养，并通过商业酶免疫测定法（EIA）检测仔猪粪便中A/B毒素的存在情况。艰难梭菌分离株通过双位点（DLST）和多位点序列分型（MLST）进行分型。从15.5%的仔猪和31.7%的啮齿动物中分离出了艰难梭菌。大多数分离株被鉴定为DLST型4-4和17-5（均为ST11），在啮齿动物和仔猪中均有发现。本研究结果表明，啮齿动物可能在艰难梭菌菌株向猪的传播和扩散中起作用。
