从绵羊中培养角膜内皮细胞。
Cultivation of corneal endothelial cells from sheep.
机构信息
Queensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland, 4101, Australia; School of Biomedical Science, Queensland University of Technology, 2 George Street, Brisbane, Queensland, 4001, Australia; King Faisal University, Hofuf, Saudi Arabia.
Queensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland, 4101, Australia; School of Biomedical Science, Queensland University of Technology, 2 George Street, Brisbane, Queensland, 4001, Australia; The University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, 4029, Australia.
出版信息
Exp Eye Res. 2018 Aug;173:24-31. doi: 10.1016/j.exer.2018.04.011. Epub 2018 Apr 20.
Research is currently under way to produce tissue engineered corneal endothelium transplants for therapeutic use in humans. This work requires the use of model animals, both for the supply of corneal endothelial cells (CECs) for experimentation, and to serve as recipients for test transplants. A variety of species can be used, however, a number of important advantages can be gained by using sheep as transplant recipients. The purpose of the present study was therefore to develop a method for culturing sheep CECs that would be suitable for the eventual construction of corneal endothelium grafts destined for sheep subjects. A method was established for culturing sheep CECs and these were compared to cultured human CECs. Results showed that cultured sheep and human CECs had similar growth characteristics when expanded from corneal endothelium explants on gelatin-coated plates, and achieved similar cell densities after several weeks. Furthermore, the markers zonula occludens-1, N-cadherin and sodium potassium ATPase could be immunodetected in similar staining patterns at cell boundaries of cultured CECs from both species. This work represents the first detailed study of sheep CEC cultures, and is the first demonstration of their similarities to human CEC cultures. Our results indicate that sheep CECs would be an appropriate substitute for human CECs when developing methods to produce tissue engineered corneal endothelium transplants.
目前正在进行研究，以生产用于人类治疗的组织工程角膜内皮移植体。这项工作需要使用模型动物，既为实验提供角膜内皮细胞（CEC），也为测试移植提供受体。可以使用多种物种，但是，使用绵羊作为移植受体可以带来许多重要的优势。因此，本研究的目的是开发一种适合最终构建用于绵羊受试者的角膜内皮移植物的方法，用于培养绵羊 CEC。建立了一种培养绵羊 CEC 的方法，并将其与培养的人 CEC 进行了比较。结果表明，从明胶包被的平板上培养的角膜内皮外植体中扩增的培养绵羊和人 CEC 具有相似的生长特性，并且经过数周后达到相似的细胞密度。此外，在两种物种的培养 CEC 的细胞边界处，可以用免疫检测到类似的染色模式的紧密连接蛋白-1、N-钙黏蛋白和钠钾 ATP 酶。这项工作代表了对绵羊 CEC 培养的首次详细研究，也是首次证明其与人 CEC 培养的相似性。我们的结果表明，在开发用于生产组织工程角膜内皮移植物的方法时，绵羊 CEC 将是人类 CEC 的合适替代品。
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