Department of Biochemistry and Bioinformatics, Dr. MGR Janaki College of Arts and Science for Women, Affiliated to University of Madras, Chennai, 600028, Tamilnadu, India; Department of Biochemistry, Kongunadu Arts and Science College, Affiliated to Bharathiar University, Coimbatore, 641029, Tamil Nadu, India.
Department of Biochemistry, Kongunadu Arts and Science College, Affiliated to Bharathiar University, Coimbatore, 641029, Tamil Nadu, India.
Biomed Pharmacother. 2018 Jul;103:719-728. doi: 10.1016/j.biopha.2018.04.047. Epub 2018 Apr 24.
Moringa concanensis Nimmo is a medicinal plant for treating various human illnesses including menstrual pain, high blood pressure, jaundice, inflammation, pain, fever, sore eyes, and cholesterol in Indian folk medicine. Despite its versatility, its antihyperglycemic mechanism of action (in vitro and in vivo) remains unclear. Therefore, in this study we developed the possible antihyperglycemic mechanism of action in 3T3-L1 cells by evaluating mRNA and protein expression, which are associated with adipogenesis and lipogenesis (insulin sensitizer). Also, the antihyperglycemic activity of the ethanolic extract of M. concanensis Nimmo leaves (EEMCN) was evaluated on glucose, insulin, biochemical, and lipid profile in experimental diabetic rat models induced with streptozotocin (STZ). Results showed that EEMCN leaves enhanced lipid accumulation in 3T3-L1 cells, as assessed by Oil Red O staining, and upregulated gene expression level of PPAR-γ, C/EBP-α, t-SREBP, FAS, Glut-4, adipogenin, DAG, and LPL through Akt signaling in 3T3-L1 cells. Also, EEMCN treatment increased body weight and insulin level and lowered blood glucose, HbA1c, amylase, and lipid profile level in STZ-induced diabetic rats. In conclusion, EEMCN possesses in vivo antidiabetic potential, having such efficacy through a mechanism of action that involves antihyperglycemic, hypoglycemic, and potential insulin sensitizer (PPAR-γ, C/EBP-α/Akt over expression) action.
辣木叶是印度民间医学中治疗各种人类疾病的药用植物，包括月经痛、高血压、黄疸、炎症、疼痛、发烧、眼痛和胆固醇。尽管它用途广泛，但它在体外和体内的降血糖作用机制尚不清楚。因此，在这项研究中，我们通过评估与脂肪生成和脂生成（胰岛素增敏剂）相关的 mRNA 和蛋白质表达，来研究辣木叶的可能降血糖作用机制。此外，还评估了辣木叶的乙醇提取物（EEMCN）对链脲佐菌素（STZ）诱导的实验性糖尿病大鼠模型的葡萄糖、胰岛素、生化和脂质谱的降血糖活性。结果表明，EEMCN 叶片通过 Akt 信号通路增强了 3T3-L1 细胞中的脂质积累，如油红 O 染色评估的那样，并上调了 3T3-L1 细胞中 PPAR-γ、C/EBP-α、t-SREBP、FAS、Glut-4、 adipogenin、DAG 和 LPL 的基因表达水平。此外，EEMCN 处理增加了体重和胰岛素水平，并降低了 STZ 诱导的糖尿病大鼠的血糖、HbA1c、淀粉酶和脂质谱水平。总之，EEMCN 具有体内抗糖尿病潜力，其作用机制涉及降血糖、降血糖和潜在的胰岛素增敏剂（PPAR-γ、C/EBP-α/Akt 过表达）作用。
