Matsuoka Izumi, Hata Kanae, Katsuzaki Hirotaka, Nakayama Hiroko, Zang Liqing, Ota Mizuho, Kim Youngil, Chu Djong-Chi, Juneja Lekh Raj, Nishimura Norihiro, Shimada Yasuhito
Graduate School of Regional Innovation Studies Mie University Tsu Japan.
Graduate School of Bioresources Mie University Tsu Japan.
Food Sci Nutr. 2022 Feb 11;10(4):1248-1256. doi: 10.1002/fsn3.2758. eCollection 2022 Apr.
The zebrafish obesogenic test (ZOT) is a powerful tool for identifying anti-adipogenic compounds for in vivo screening. In our previous study, we found that (MO) leaf powder suppressed the accumulation of visceral adipose tissue (VAT) in ZOT. MO demonstrates a wide range of pharmacological effects; however, little is known about its functional constituents. To identify the anti-adipogenic components of MO leaves, we prepared extracts using different extraction methods and tested the obtained extracts and fractions using ZOT. We found that the dichloromethane extract and its hexane:EtOAc = 8:2 fraction reduced VAT accumulation in young zebrafish fed a high-fat diet. We also performed gene expression analysis in the zebrafish VAT and found that CCAAT/enhancer-binding protein beta and CCAAT/enhancer-binding protein delta (associated with early stages of adipogenesis) gene expression was downregulated after fraction 2 administration. We identified a new MO fraction that suppressed VAT accumulation by inhibiting early adipogenesis using the ZOT. Phenotype-driven zebrafish screening is a reasonable strategy for identifying bioactive components in natural products.
斑马鱼致肥胖测试（ZOT）是一种用于体内筛选抗脂肪生成化合物的强大工具。在我们之前的研究中，我们发现（MO）叶粉可抑制ZOT中内脏脂肪组织（VAT）的积累。MO具有广泛的药理作用；然而，对其功能成分了解甚少。为了鉴定MO叶的抗脂肪生成成分，我们使用不同的提取方法制备了提取物，并使用ZOT对所得提取物和馏分进行了测试。我们发现二氯甲烷提取物及其己烷：乙酸乙酯 = 8:2馏分可减少高脂饮食喂养的幼龄斑马鱼的VAT积累。我们还对斑马鱼VAT进行了基因表达分析，发现给药馏分2后，与脂肪生成早期阶段相关的CCAAT/增强子结合蛋白β和CCAAT/增强子结合蛋白δ基因表达下调。我们使用ZOT鉴定了一种通过抑制早期脂肪生成来抑制VAT积累的新MO馏分。基于表型的斑马鱼筛选是鉴定天然产物中生物活性成分的合理策略。
