Ng Y C, Schifferli J A, Walport M J
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Clin Exp Immunol. 1988 Mar;71(3):481-5.
We performed experiments to investigate whether immune complexes opsonized with C3b and iC3b transferred from CR1 on one erythrocyte to CR1 on others, and studied the effect of variation in erythrocyte CR1 number on the transfer reaction. We used populations of cells of different blood groups to study this phenomenon which were separated by differential agglutination with monoclonal anti-group antibodies. The rate of transfer of immune complexes between erythrocytes was related to CR1 concentration of both donor and recipient cells; fastest transfer occurred from donor cells of low CR1 numbers to recipient cells of high CR1. These results were not explained by a difference in the binding constant of immune complexes to erythrocytes bearing different numbers of CR1. In the absence of factor I, complexes partitioned between erythrocytes according to their relative concentrations of CR1 with no release of complexes into solution. In serum, the proportion of complexes bound to donor and recipient erythrocytes was similarly related to their respective CR1 numbers with progressive release of complexes into solution. Erythrocyte CR1 may act as a dynamic buffering system which prevents immune complexes that have bound complement from fixing to vascular endothelium.
我们进行了实验，以研究用C3b和iC3b调理的免疫复合物是否会从一个红细胞上的CR1转移到其他红细胞上的CR1，并研究红细胞CR1数量变化对转移反应的影响。我们使用不同血型的细胞群体来研究这种现象，这些细胞群体通过用单克隆抗血型抗体进行差异凝集来分离。免疫复合物在红细胞之间的转移速率与供体细胞和受体细胞的CR1浓度有关；最快的转移发生在CR1数量低的供体细胞到CR1数量高的受体细胞之间。这些结果不能用免疫复合物与携带不同数量CR1的红细胞的结合常数差异来解释。在没有I因子的情况下，复合物根据红细胞相对CR1浓度在红细胞之间分配，没有复合物释放到溶液中。在血清中，与供体和受体红细胞结合的复合物比例同样与它们各自的CR1数量有关，复合物会逐渐释放到溶液中。红细胞CR1可能作为一个动态缓冲系统，防止已结合补体的免疫复合物固定到血管内皮上。
